蛋白质芯片技术检测血清室间隔缺损特异标志物的研究

目的应用蛋白质芯片技术筛选单纯性室间隔缺损血清特异标志物。方法采用病例对照设计。用CMIO蛋白质芯片检测56例单纯性室间隔缺损患儿与85例儿科常见痛患儿血清，筛选血清差异蛋白。结果有21个蛋白具有统计学差异．其中质荷比6640、784、8571，079、6441、972、8698．804、3322．549、2128．960、2291、255、2055，652、2243．846和2．99．661等10个蛋白在VSD患儿血清中呈高表达，而质荷比2050．946、9306．537、7783．535、9198．666、2143．745、4604．572、7993．109、2824．602、2230．218、2083．311和9360．675等11个蛋白下调。结论蛋白质芯片技术是进行室间隔缺损分子流行病学研究的有效工具，所筛选出的蛋白成分可能是单纯性室间隔缺损的血清标志物。

Detection of serum biomarkers from isolated ventricular septal defect with protein chip technology

Objective To screen serum biomarkers from sera of ventricular septal defect (VSD) children by using proteinChip. Methods With a case-control design, serum samples from 56 isolated VSD patients and 85 patients with common pediatric diseases were detected with CM10 ProteinChip for the protein biomarkers that were significantly different from that in sera of VSD children and control. Results The contents of 21 proteins were significantly different from that of sera of VSD children and controls. Ten of the 21 proteins with the mass/ charge (m/z) of 6640.784, 8571.079, 6441.972, 8698.804, 3322.549, 2128.960, 2291.255, 2055.652, 2243.846 and 2.99.661 were highly expressed in sera of VSD children, and 11 proteins with m/z 2050.946, 9306.537, 7783.535, 9198.666, 2143.745, 4604.572, 7993.109, 2824.602, 2230.218, 2083.311 and 9360.675 were down-regulated in sera of VSD children. Conclusion ProteinChip technology is a effective tool for molecularly epidemiological study on VSD patients. The screened proteins are likely to be the biomarkers in sera of VSD patients.