Immunomodulator ‘mushroom beta glucan’ induces Wnt/β catenin signalling and improves wound recovery in tilapia and rat skin: a histopathological study |
| |
| Authors: | Chien‐Mei Hsiao Yu‐Sheng Wu Fan‐Hua Nan Shih‐Ling Huang Lynette Chen Shiu‐Nan Chen |
| |
| Affiliation: | 1. Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan;2. Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan;3. Freshwater Aquaculture Research Center, Fisheries Research Institute, Council of Agriculture, Changhua, Taiwan;4. Oregon Health and Science University Hospital Oregon, Oregon, OR, USA;5. College of Life Science, National Taiwan University, Taipei, Taiwan |
| |
| Abstract: | ![]()
The present study aims to investigate the effects of mushroom beta glucan (MBG) on wound recovery in partial hepatectomy (PH) in Nile tilapia (Oreochromis niloticus) and in rat skin wound healing examination. Following PH, we focussed on the effects on liver repair ability using in vitro and in vivo tests. In vitro, we examined whether the MBG has an impact on liver cell proliferation, mainly through 3‐(4,5‐dimethylthiazolyl‐2)‐2,5‐diphenyltetrazolium bromide (MTT) assays and bromodeoxyuridine (BrdU) cell proliferation assay detection method. Results showed that MBG treatment was remarkable in enhancing cell proliferation of hepatocytes and in maintaining the cellular viability. Immunohistochemical staining to analyse Wnt/β‐catenin signalling also showed that MBG has the effect of promoting cell proliferation of liver tissues after PH surgery. |
| |
| Keywords: | Growth factor Histopathology Immunomodulator Wnt/β ‐catenin signalling Wound recovery |
|
|
