| 白纹伊蚊和埃及伊蚊defensin A基因克隆及序列分析 |
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| 引用本文： | 刘先凯,赵彤言,朱礼华,董言德,陆宝麟.白纹伊蚊和埃及伊蚊defensin A基因克隆及序列分析[J].寄生虫与医学昆虫学报,2002,9(4):216-221. |
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| 作者姓名： | 刘先凯 赵彤言 朱礼华 董言德 陆宝麟 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,北京,100071 |
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| 基金项目： | 中国人民解放军总后勤部“九五”医学杰出青年基金课题资助 |
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| 摘 要： | 应用PCR技术从白纹伊蚊和埃及伊蚊基因组中扩增出defensinA基因 ,并与文献报道的defensinA的5个型的cDNA序列进行同源性比较 ,发现此两序列中存在内元 ;从埃及伊蚊体内扩增的片段为蚊虫defen sinAl的前体AaDefAl;从白纹伊蚊体内扩增的片段为defensinA的 1个新型 ,命名为DefA6。
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| 关 键 词： | 白纹伊蚊 埃及伊蚊 昆虫防御素 基因 cDNA序列 |
| 修稿时间： | 2001年6月12日 |
CLONING AND SEQUENCING OF THE DEFENSIN A GENE FROM AEDES AEGYPTI AND AE.ALBOPICTUS |
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| Liu Xiankai,Zhao Tongyan,Zhu Lihua,Dong Yande,Lu Baolin.CLONING AND SEQUENCING OF THE DEFENSIN A GENE FROM AEDES AEGYPTI AND AE.ALBOPICTUS[J].Acta Parasitologica et Medica Entomologica Sinica,2002,9(4):216-221. |
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| Authors: | Liu Xiankai Zhao Tongyan Zhu Lihua Dong Yande Lu Baolin |
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| Abstract: | Defensin A gene was amplified from genome of Ae.aegypti and Ae.albopictus. The analysis of homogeneity compared with the five cDNA sequnces of reported defensin A indicated there was an intron in the two sequences respectively.The sequence amplified from Ae.aegypti was the precursor of AaDef Al,the noval isoform of defensin A gene amplified from Ae.albopictus was named Def A6. |
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| Keywords: | Aedes aegypti Aedes albopictus Insect defensin Gene cDNA sequence |
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