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Light-emitting diodes and half-cell electrodes in experimental recording of electroretinogram c-wave
Authors:Marko Hawlina MD  Peter L G De Villiers
Institution:(1) Vision Research Unit of Sherrington School (UMDS), The Rayne Institute, St Thomas' Hospital, SE1 7EH London, England;(2) University Eye Clinic, Medical Centre Ljubljana, Ljubljana, Slovenia;(3) University Eye Clinic, 61000 Ljubljana, Slovenia
Abstract:A method of electroretinogram c-wave recording in the rat was developed that uses a contact lens electrode connected through a saline bridge with a silver-silver chloride half-cell. A cluster of light-emitting diodes, regulated by an electronic light-emitting diode stimulator, was used as a light source. The method enables recordings of c-waves of 0.4–1.6 mV amplitude as well as other electroretinogram components in narrow limits of variation. The two main sources of response variability are voltage shunting and eyeball protrusion.
Keywords:Eelctroretinogram c-wave  electrodes  light emitting diodes  rat
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