Plexin-B2 在人乳腺癌中的表达及临床意义*

目的:探讨人乳腺癌中Plexin-B 2 的表达，以及其与人表皮生长因子受体-2（Her-2）共表达与乳腺癌恶性行为的关系。方法:免疫组化SP法检测15例正常乳腺组织中Plexin-B 2 蛋白的表达，及112 例乳腺癌组织中Plexin-B 2 和Her-2 蛋白的表达。结果:Plexin-B 2 在癌细胞和正常乳腺组织小叶及导管上皮细胞中的阳性表达率分别为69.64% 和60.00% ，差异无统计学意义（P>0.05）。 Plexin-B 2 在乳腺癌组织癌周微脉管中的阳性表达率为44.64% ，而正常乳腺微脉管均为阴性。癌细胞和癌周微脉管中Plexin-B 2 的表达正相关（r=0.593，P=0.000），并且都与临床分期及淋巴结转移有关（P<0.05）。 Plexin-B 2、Her-2 共表达比Plex in-B 2 单阳性的肿瘤临床分期晚、淋巴结转移率高，差异有统计学意义（P<0.05）。 结论:Plexin-B 2 异常表达可能与乳腺癌的恶性进展有关，Plexin-B 2-Her- 2 共表达可能促使乳腺癌的侵袭转移。 

Expression and Significance of Plexin-B2 in Human Breast Cancer

Objective:To explore the expression and significance of Plexin-B2 in human breast cancer and to determine whether its co-expression with human epidermal growth factor receptor 2 (Her-2) is correlated with tumor aggressiveness. Methods:The pro-tein expression of Plexin-B2 and Her- 2 was investigated by immunohistochemistry SP method among 112 cases of breast carcinoma. The protein expression of Plexin-B2 was also detected among15cases of normal breast tissues. Results: There was no significant dif-ference in the expression of Plexin-B 2 between the breast carcinoma cells and the epithelial cells of the normal breast tissue from mam -mary lobules and ducts ( P > 0.05); their expression rates were 69.64% and60.00%, respectively. The positive expression of Plexin-B2 was detected in44.62% of pericancerous microvessels in the breast cancer tissues, whereas no Plexin-B2 expression was observed in the microvessels of the normal breast tissues. There was a positive relationship in Plexin-B 2 expression between the tumor cells and the pericancerous microvessels in human breast cancer ( r = 0.593, P = 0.000 ), which were correlated with clinical stage and lymph node metastases ( P < 0.05). Plexin-B 2 expression in the tumor cells was also associated with the histologic grade ( P < 0. 05). The tumors that co-expressed Plexin-B 2 and Her- 2 were characterized by worse staging and higher incidences of lymph node metastases than those that express Plexin-B 2 alone; the difference was significant ( P < 0. 05). Conclusion:The aberrant expression of Plexin-B 2 may be as-sociated with the malignant progression of breast cancer. Moreover, Plexin-B2 may contribute to the invasiveness and metastatic behav-ior of tumors when co-expressed with Her-2.