原卟啉钠对急性肝损伤小鼠肝组织SOD、MDA的影响

 目的 探讨原卟啉钠对四氯化碳（CCl4）致急性肝损伤小鼠血清转氨酶和肝组织超氧化物歧化酶（SOD）、脂质过氧化产物丙二醛（MDA）的影响。方法 60只ICR小鼠随机分为正常对照组、CCl4模型组、联苯双酯组、原卟啉钠低、中、高剂量组。各治疗组每天灌胃给药及造模16h后，摘眼球取血测定血清中谷丙转氨酶（ALT）和谷草转氨酶（AST）活性，剖腹取肝测定肝脏SOD活力和MDA含量。结果 CCl4模型组小鼠血清ALT和AST活力分别为（1879±1219）、（2210±1585）U/L，与正常对照组比较，降低显著（P<0.01）；联苯双酯组、原卟啉钠低、中、高剂量组的SOD活力和MDA含量分别为（207.61±16.02）、（184.35±13.42）、（190.88±17.77）、（199.38±14.43）U/mgprot和（1.08±0.15）、（1.35±0.26）、（1.07±0.16）、（0.92±0.18）nmol/mgprot，与CCl4模型组比较，差异有统计学意义（P<0.05～P<0.01）。结论 原卟啉钠能有效阻止CCl4致急性肝损伤小鼠肝组织SOD活性降低，脂质过氧化产物MDA含量升高，具有一定的保肝降酶作用。

Effect of Protoporphyrin Disodium on SOD and MDA in Liver Tissue of Mice with Acute Hepatic Injury

Objective To study the effect of protoporphyrin disodium (NAPP) on activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver tissue of mice with acute hepatic injury induced by carbon tetrachloride (CCl4). Methods 60 ICR mice were randomly divided into normal control group, CCl4 model group, bifendate pills group, NAPP low dose group, NAPP medium dose group and NAPP high dose group. Mice of each treatment group were injected different drugs by intragastric administration continuously and then a single dose of CCl4 was given to establish acute hepatic injury model. 16 hours later, blood was collected by picking eyeballs to detect the activities of ALT and AST in serum, liver was taken out to detect the activity of SOD and the content of MDA in liver tissiue of each group. Results Compared with the normal control group, the activities of ALT and AST in serum of the CCl4 model group were (1879±1219) U/L and (2210±1585) U/L, there has a significant difference (P<0.01); The activities of SOD and MDA in the liver tissues of the bifendate pills group, NAPP low dose group, NAPP medium dose group and NAPP high dose group were (207.61±16.02), (184.35±13.42), (190.88±17.77), (199.38±14.43) U/mgprot and (1.08±0.15), (1.35±0.26), (1.07±0.16), (0.92±0.18) nmol/mgprot, respectively, which have statistical sense compared with model group (P<0.05～P<0.01). Conclution NAPP can effectively prevent the decrease of SOD and increase of MDA in liver tissues of mice with acute hepatic injury, this shows NAPP has a good effect of protecting liver and decreasing enzymes on mice with acute hepatic injury induced by CCl4.