视网膜母细胞瘤基因治疗的实验研究

目的观察外源性Ｒｂ基因在视网膜母细胞瘤（ｒｅｔｉｎｏｂｌａｓｔｏｍａ，ＲＢ）移植瘤内的表达情况，及其对ＲＢ移植瘤生长的影响，为进行ＲＢ体内基因治疗提供实验依据。方法建立裸鼠眼玻璃体腔ＲＢ移植瘤模型，构建Ｒｂ基因的逆转录病毒表达载体，用脂质体将Ｒｂ基因导入裸鼠ＲＢ移植瘤，免疫组织化学染色及流式细胞仪间接免疫荧光法检测Ｒｂ基因表达，裸鼠ＲＢ移植瘤生长的活体观察、分级、组织病理形态观察。结果脂质体Ｄｏｓｐｅｒ介导的Ｒｂ基因转染可在ＲＢ移植瘤内表达至少７天，并部分抑制ＲＢ移植瘤的生长，抑制效果与Ｒｂ蛋白表达量、治疗时机有关。结论外源性Ｒｂ基因可在ＲＢ移植瘤内表达并部分抑制ＲＢ移植瘤的生长。

Experimental research on in vivo genetherapy of retinoblastoma

Objective To investigate the expression condition of exogenous Rb gene in nude mouse vitreous transplantated retinoblastoma(RB) and its influence on the growth of the RB tumor in vivo. MethodsZEstablishing the model of RB transplantation in nude mouse vitreous, followed by constructing the retrovirus vector of Rb gene and transfecting it into the model by using liposome. The expression condition of Rb gene was analyzed by immuohistochemistry and flow cytometry. The growth condition of the RB transplantation tumor was examined ophthalmologically and pathologically. ResultsThe exogenous Rb gene expressed in the RB transplantation tumor for at least 7 days and partially suppressed the growth of the RB tumor. The level of suppression was related with Author the amount of the Rb protein expressed and the time to transfect the Rb gene after the RB transplantation tumor was established. Conclusion The exogenous Rb gene can express in the RB transplantation tumor and can partially suppress the growth of the RB tumor in vivo.