Box-Behnken设计-响应面法优选雪松松针中蛇床子素的提取工艺

目的:优选雪松松针中蛇床子素的提取工艺。方法:采用高效液相色谱法测定蛇床子素含量。在单因素考察的基础上,以乙醇体积分数、提取时间、料液比为考察因素,以蛇床子素含量为响应值,采用Box-Behnken设计-响应面法优选雪松松针中蛇床子素的提取工艺,并进行验证试验。结果:确定的最佳提取工艺为乙醇体积分数88%,料液比1∶20(g/mL),提取2次、每次57min。在此工艺条件下,提取得到蛇床子素的平均含量为0.675 7 mg/g(RSD=1.78%,n=3),与预测值0.680 9 mg/g的相对误差为0.59%。结论:优选的最佳提取工艺方法简便、可行,可用于雪松松针中蛇床子素的提取。

Optimization of Extraction Technology of Osthole from Pine Needles of Cedrus deodara by Box-Behnken Design-response Surface Methodology

OBJECTIVE:To optimize the extraction technology of osthole from the pine needles of Cedrus deodara.METHODS:HPLC method was adopted to determine the content of osthole.Based on single factor test,ethanol volume fraction,extraction time and material-solvent ratio were selected as influential factors,and the content of osthole was selected as response value.Box-Behnken design-response surface methodology was used to optimize the extraction technology of osthole in pine needles of C.deodara.Validation test was conducted.RESULTS:The optimal extraction technology was as follows as ethanol volume fraction of 88%,material-solvent ratio of 1∶20(g/mL),extracting for 2 times,lasting for 57 min each time.Under this technology,average content of osthole was 0.675 7 mg/g(RSD=1.78%,n=3),and the relative error of which to predicted value 0.680 9 mg/g was 0.59%.CONCLUSIONS:The optimal extraction technology is simple and feasible,and it can be used for the extraction of osthole from the pine needles of C.deodara.