| 水飞蓟宾脂质体包封率及其水飞蓟宾含量测定 |
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| 引用本文： | 余江南,徐希明,朱源,高昊.水飞蓟宾脂质体包封率及其水飞蓟宾含量测定[J].中国中药杂志,2003,28(11):1027-1030. |
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| 作者姓名： | 余江南 徐希明 朱源 高昊 |
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| 作者单位： | 江苏大学,药学院,江苏,镇江,212001 |
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| 基金项目： | 江苏省自然科学基金资助项目 (BK 2 0 0 2 0 0 7)，江苏高校高新技术产业化项目 (JH 0 1 0 6 1 ) |
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| 摘 要： | 目的 :建立一种一阶导数紫外分光光度法测定水飞蓟宾脂质体包封率及脂质体中水飞蓟宾含量。方法 :水飞蓟宾脂质体溶液经SephadexG100柱分离后 ,以水飞蓟宾一阶导数紫外扫描图谱在 277nm和 294nm处出现的特征峰和特征谷的振幅ΔA进行定量。结果 :方法的线性范围为 0.5～30.0mg·L-1(r=0.9998) ,回收率为10 1.7% ,精密度为 1.8%。采用该法测定药脂比为 0.02～0.06的水飞蓟宾脂质体 ,包封率为 65.1%～83.0% ,脂质体中水飞蓟宾平均含量为 760 .4mg·L-1(n=4)。结论 :一阶导数分光光度法准确、简便 ,可用于水飞蓟宾脂质体的质量控制。
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| 关 键 词： | 水飞蓟宾脂质体 包封率 含量测定 一阶导数分光光度法 |
| 文章编号： | 1001-5302(2003)11-1027-04 |
| 收稿时间： | 2003/8/15 0:00:00 |
| 修稿时间： | 2003年8月15日 |
Determination of Concentration and Encapsulation Efficie ncy of Silybin Liposomes |
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| YU Jiang-nan;XU Xi-ming;ZHU Yuan;GAO Hao.Determination of Concentration and Encapsulation Efficie ncy of Silybin Liposomes[J].China Journal of Chinese Materia Medica,2003,28(11):1027-1030. |
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| Authors: | YU Jiang-nan;XU Xi-ming;ZHU Yuan;GAO Hao |
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| Institution: | School of Pharmacy, Jiangsu University, Zhenjiang 212001, Jiangsu, China. |
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| Abstract: | OBJECTIVE: To determine the encapsulation efficiency of silybin liposomes. METHOD: After Silybin liposomes solution was separated through sphadex G-100 column, eluent collected was determined by the first derivative UV spectrometry. RESULT: The calibration curve of Silybin was linear in the range of 0.5-30.0 mg x L(-1) (r = 0.9998). The average recovery was 101.7%. The relative standard deviation was 1.8%. The results showed that the encapsulation efficiency ranged from 65.1%-83.0% when the drug/phospholipid ratio varied from 0.02-0.06. The concentration of SLB in liposomes prepared by optimum technique was 760.4 mg x L(-1) (n = 4). CONCLUSION: The method is accurate, simple and suitable for the quality control of Silybin liposomes. |
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| Keywords: | silybin liposomes encapsulation efficie ncy assay first derivative UV spectrometry |
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