| 蝎毒对人肝癌Bel-7404 细胞端粒酶活性的影响 |
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| 引用本文： | 李桂生,杨春旭,韦晓谋,朱灵. 蝎毒对人肝癌Bel-7404 细胞端粒酶活性的影响[J]. 东南大学学报(医学版), 2004, 23(3): 166-169 |
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| 作者姓名： | 李桂生 杨春旭 韦晓谋 朱灵 |
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| 作者单位： | 广西医科大学第四附属医院,肿瘤科,广西,柳州,545005;广西医科大学第四附属医院,生物技术研究所,广西,柳州,545005 |
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| 基金项目： | 广西壮族自治区科技厅资助项目(9920028). |
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| 摘 要： | 目的：观察蝎毒(BMK)对人肝癌Bel-7404细胞端粒酶活性的影响。方法：不同浓度的BMK处理体外培养的人肝癌Bel-7404细胞72h,聚合酶链反应一端粒重复扩增(PCR-TRAP)法测定端粒酶活性,四甲基偶氮唑盐比色(MTT)法测定细胞增殖抑制。结果：BMK可抑制Bel-7404细胞端粒酶活性,随BMK浓度增大,端粒酶活性抑制增强。MTT结果显示BMK抑制Bel-7404细胞增殖且抑制有浓度依赖性。结论：端粒酶活性的抑制可能是BMK发挥抗癌活性的作用机制之一,并可作为评价BMK抗肝癌冶疗效果的指标之一。
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| 关 键 词： | 蝎毒 肝癌 端粒酶 |
| 文章编号： | 1671-6264(2004)03-0166-04 |
| 修稿时间： | 2003-10-13 |
Effect of buthus martensii kirsch on telomere enzymatic activity of liver cancer Bel-7404 cell |
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| LI Gui-sheng ,YANG Chun-xu ,WEI Xiao-mou ,ZHU Ling. Effect of buthus martensii kirsch on telomere enzymatic activity of liver cancer Bel-7404 cell[J]. Journal of Southeast Univ: Medical Sci Ed, 2004, 23(3): 166-169 |
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| Authors: | LI Gui-sheng YANG Chun-xu WEI Xiao-mou ZHU Ling |
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| Affiliation: | LI Gui-sheng 1,YANG Chun-xu 1,WEI Xiao-mou 2,ZHU Ling 2 |
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| Abstract: | Objective To observe the effect of buthus martensii kirsch (BMK) on liver cancer Bel?7404 cell telomere enzymatic activity.Methods Liver cancer Bel?7407 cells cultured in vitro were exposed to different concentrations of BMK over 72 hours and their enzymatic activity was measured using polymerase chain reaction-telomere repeated amplification (PCR?TRAP). Meanwhile cell proliferation suppression was measured using MTT. Results BMK could suppress telomere enzymatic activity of liver cancer Bel?7404 cells in a dose-dependent manner further more MTT results showed BMK suppresses Bel?7404 cell proliferation in a dose-dependent manner.Conclusion Telomere activity suppression is one mechanism of BMK anti-cancer activity, which could be one index to evaluate the effect of BMK in treating liver cancer. |
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| Keywords: | buthus martensii kirsch liver cancer telomere enzyme |
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