| 风疹病毒荧光逆转录-聚合酶链反应快速检测方法的建立 |
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| 引用本文： | 吴华森,高筱萍,徐昌平,卢亦愚,冯燕. 风疹病毒荧光逆转录-聚合酶链反应快速检测方法的建立[J]. 中国疫苗和免疫, 2010, 0(1) |
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| 作者姓名： | 吴华森 高筱萍 徐昌平 卢亦愚 冯燕 |
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| 作者单位： | 浙江中医药大学;浙江省疾病预防控制中心浙江省公共卫生应急检测关键技术重点实验室; |
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| 摘 要： |
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| 关 键 词： | 风疹病毒 荧光逆转录-聚合酶链反应 检测 TaqMan探针 |
Development of the Real-time Reverse Transcription-polymerase Chain Reaction Assay for the Rapid Detection of Rubella Virus |
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| WU Hua-sen,GAO Xiao-ping,XU Chang-ping,et al. Development of the Real-time Reverse Transcription-polymerase Chain Reaction Assay for the Rapid Detection of Rubella Virus[J]. Chinese Journal of Vaccines and Immunization, 2010, 0(1) |
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| Authors: | WU Hua-sen GAO Xiao-ping XU Chang-ping et al |
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| Affiliation: | WU Hua-sen,GAO Xiao-ping,XU Chang-ping,et al(Zhejiang Chinese Medical University,Hangzhou 310051,Zhejiang,China) |
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| Abstract: | Objective A new TaqMan probe based real-time assay was developed to rapid detection of rubella virus.Methods The specific primer pair and probe were designed within the conserved P150 gene of rubella virus and the PCR reactive condition was optimized to improve the sensitivity and specificity of the assay.Clinical specimens collected from two outbreaks were detected by the developed assay.Results The assay is specific to detect rubella virus.There is no cross-reactions to measles,mumps,influenza and other r... |
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