强志怡神胶囊的大孔树脂纯化工艺优选

目的:优选强志怡神胶囊的大孔树脂纯化工艺。方法:以细叶远志皂苷、芍药苷、甘草酸、甘草苷的比吸附量和洗脱率为指标筛选大孔树脂型号。采用单因素试验考察上样液质量浓度、径高比、洗脱剂体积等因素对强志怡神胶囊大孔树脂纯化工艺的影响。结果:选用HPD-100型大孔树脂,最佳纯化工艺为上样液质量浓度0.1 g·m L-1,树脂-生药量(3∶1),树脂径高比1∶7.5,上样速度3 BV·h-1,加4 BV水洗除杂,继加70%乙醇5 BV洗脱,洗脱速度3 BV·h-1。细叶远志皂苷、芍药苷、甘草酸、甘草苷的转移率分别为78.55%,88.62%,82.47%,83.15%,得膏率7.52%,干燥时间0.5 h。结论:该工艺能有效去除远志、白芍、甘草水提液中的淀粉、糖类等杂质,所得纯化物易于制剂成型,可作为强志怡神胶囊的纯化工艺。

Optimization of Purification Process of Qiangzhi Yishen Capsules with Macroporous Absorptive Resin

Objective: To optimize purification process of Qiangzhi Yishen capsules with macroporous resin. Method: Absorption ratio and elution rate of tenuifolin, paeoniflorin, glycyrrhizic acid and liquiritin were taken as indexes to screen out types of macroporous resins.Effects of the concentration of solution, diameter-height ratio and eluent volume on purification process of Qiangzhi Yishen capsules were investigated by single factor tests. Result: HPD-100 macroporous resin was selected, optimum purification process was as following:the mass concentration of sample loading solution 0.1 g· mL^-1, resin-material drug (3:1), diameter-height ratio of resin 1:7.5, sample loading speed 3 BV· h^-1, decontaminated with 4 BV of water, and then eluted with 5 BV of 70% ethanol, elution speed of 3 BV· h^-1.Transport rates of tenuifolin, paeoniflorin, glycyrrhizic acid and liquiritin were 78.55%, 88.62%, 82.47%, 83.15%, with rate of extract of 7.52% and drying time of 0.5 h. Conclusion: This process can effectively remove starch, saccharides and other impurities in water extract, these purified substances is easy to use in preparations.