活血化瘀类方干预急性心肌缺血犬心肌组织病理改变及心肌细胞凋亡的实验研究

目的：观察活血化瘀类方对心肌细胞凋亡干预作用。方法：采用结扎冠状动脉左前降支的方法复制急性心肌缺血模型，35条犬随机分成对照组、心肌缺血组、丹参饮组、血府逐瘀汤组、失笑散组、活络效灵丹组、桃红四物汤组，每组5条。采用HE染色及DNA末端标记法(TUNEL)分别观察心肌组织病理变化与心肌细胞凋亡的变化。结果：对照组无细胞变性与坏死。仅见极少量凋亡阳性细胞：心肌缺血组与失笑散组有明显心肌组织坏死，心肌细胞凋亡增多；丹参饮组、血府逐瘀汤组、活络效灵丹组、桃红四物汤组心肌细胞坏死及凋亡均显著减轻。结论：血府逐瘀汤、活络效灵丹、桃红四物汤、丹参饮可有效抑制心肌细胞坏死及凋亡．减轻心肌细胞损伤，对缺血心肌有保护作用，而失笑散对急性缺血心肌无明显保护作用。

The Effect of HuoXueHuaYu Decoctions on Myocardial Pathology and Myocardial Cellar Apoptosis of Experimental Aute Ischemic Myocardium

Objective: To explore the effect of HuoXueHuaYu decoctions on myocardial pathology and myocardial cellar apoptosis of experimental acute ischemic myocardium. Methods: Thirty-five dog were randomly divided into 7 groups control group, ischemic group, ShiXiaoSan group, XueFuZhuYu group, HuoLuoXiaoLingDan group, TaoHongSiWuTang group and DanShenYin group.The left anterior descending coronary artery was ligated to make a model of acute ischemic myocardium. The changes in myocardial pathology and cellar apoptosis were detected. Results: The number of myocardium cellar apoptosis in XueFuZhuYu group,HuoLuoXiaoLingDan group,TaoHongSiWuTang group, and DanShenYin group were significantly less than those of ischemic group and ShiXiaoSan group respectively. The number of myocyte necrotic cell increased in ischemic group and ShiXiaoSan group respectively. Conclusion: XueFuZhuYu decoction,HuoLuoXiaoLingDan decoction,TaoHongSiWuTang decoction, and DanShenYin decoction can protect ischemic myocardium.