银杏叶提取物对培养的人眼视网膜神经节细胞的保护作用

目的 探讨银杏叶提取物（EGb761）对培养的人眼视网膜神经节细胞(RGC)的保护作用。方法 对照实验研究。将培养的人眼视网膜细胞分为对照组、谷氨酸组、EGb761组及谷氨酸+EGb761组,用Thy-1作为RGC特异性荧光抗体,以免疫流式细胞技术评价EGb761对人眼RGC的保护作用。多组间细胞存活率比较采用重复测量的方差分析,组间两两比较采用LSD t检验。结果 在不同干预因素作用下,RGC存活率呈现不同变化,对照组为(61.94±7.75)％,谷氨酸组为(44.59 +4.19)％,EGb761组为(75.05 +3.90)％,EGb761+谷氨酸组为(63.19±9.44)％;各组间RGC存活率比较差异有统计学意义(F=13.329,P＜0.01)。各组与对照组RGC存活率两两比较,显示谷氨酸组RGC存活率降低(P =0.010),EGb761组RGC存活率升高(P=0.019),EGb761+谷氨酸组与对照组RGC存活率差异无统计学意义(P =0.801);与谷氨酸组相比EGb761组和EGb761+谷氨酸组RGC存活率明显升高(P=0.000,0.020)。死亡RGC中大RGC所占百分比,EGb761组为(24.63+7.21)％,EGb761+谷氨酸组为(25.99±5.05)％,与对照组(36.69±2.92)％比较,两组死亡RGC中大RGC所占百分比均降低(P=0.001,0.002);与谷氨酸组(40.78±3.34)％相比,两组大RGC死亡百分比亦降低(P =0.000,0.000)。结论 EGb761可对抗谷氨酸兴奋性毒性造成的RGC损伤,对体外培养的人眼RGC具有明显的保护作用。

The protective effects of ginkgo biloba extract on cultured human retinal ganglion cells

Objective This study was designed to investigate the protective effects of ginkgo biloba extract (EGb)761 on human retinal ganglion cells(RGC). Methods It was an experimental study.Cultured human retinal cells were separated for four groups: control group, glutamate group, EGb761 group and EGb761 + glutamate group. RGC survival rate was determined by immune Flow cytometry using Thy-1 as a special fluorescent antibody of RGC. RGC survival rate in Multi groups were analysed by repetitive measure analysis of variance (ANOVA), and the differences between groups were analysed by LSD t test. Results The effects of EGb761 on cultured human RGC were different under different interfere factors. The RGC survival rate was (61.94 + 7. 75 ) ％ in control group, (44. 59 + 4. 19 ) ％ in glutamate group, ( 75.05 ±3.90) ％ in EGb761 group, and ( 63. 19 + 9. 44 ) ％ in EGb761 + glutamate group. There were significant difference in different groups on survival rates of RG C ( F =13. 329, P ＜ 0. 01 ). Compared with the control group, the survival rate of RGC in glutamate group was significantly decreased (P =0.010), while the EGb761 group was significantly increased (P =0. 019 ), and the EGb761 + glutamate group had no significant difference ( P =0. 801 ). Compared with the glutamate group, the survival rate of RGC in the EGb761 group and the EGb761 + glutamate group was significantly increased( P =0. 000,0. 020). There was no significant difference in the percentage of the large RGC in all groups. But compared with the control group( 36. 69 + 2. 92 ) ％, the percentage of large RGC in dead RGC in the EGb761 group(24. 63 ± 7. 21 ) ％and the EGb761 + glutamate group (25. 99 ± 5.05)％ was significantly decreased (P =0.001,0.002).Compared with the glutamate group(40. 78 ±3.34)％, the death rate of the large RGC in the EGb761 group and the EGb761 + glutamate group was also significantly decreased ( P =0. 000, 0. 000). Conclusion EGb761 can protect RGC against glutamate induced neurotoxicity, and it has significant protective effect on cultured human RGC.