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| 姜黄素对缺氧/复氧心肌细胞凋亡的影响及其机制的研究 | |
| 引用本文： | 郭炳彦,韩瑞,石英辉,韩德荣,李拥军.姜黄素对缺氧/复氧心肌细胞凋亡的影响及其机制的研究[J].现代中西医结合杂志,2011,20(1):20-23. |
| 作者姓名： | 郭炳彦 韩瑞 石英辉 韩德荣 李拥军 |
| 作者单位： | 1. 河北医科大学第二医院,河北,石家庄,050000;河北省故城县医院,河北,故城,253800 2. 河北医科大学第一医院,河北,石家庄,05003 3. 河北省故城县医院,河北,故城 4. 河北医科大学第二医院,河北,石家庄,050000 |
| 摘 要： | 目的研究姜黄素(Cur)对缺氧/复氧干预下心肌细胞凋亡的影响以及其可能的作用机制。方法用体外培养的SD乳大鼠心肌细胞,建立缺氧(4 h)/复氧(1 h)模型,然后用磷脂酰肌醇3-激酶(PI3-K)特异性的抑制剂LY294002进行干预。实验分为对照组、缺氧/复氧组(H/R)、H/R+Cur组和H/R+Cur+LY294002组。用MTT比色法测定细胞活力,Annexin-V与PI双染法及流式细胞仪检测心肌细胞凋亡率,Western blotting检测磷酸化Akt(p-Akt)和总Akt(t-Akt)蛋白水平的表达变化。结果与H/R组和H/R+Cur+LY294002组相比,H/R+Cur组能显著抑制缺氧/复氧损伤所诱导的细胞存活率的下降(P均<0.01),并且可显著降低心肌细胞的凋亡率(P均<0.05);同时H/R+Cur组Akt磷酸化明显增强(P均<0.01)。结论Cur可抑制缺氧/复氧损伤引起的心肌细胞凋亡,其抗细胞凋亡作用可能与Cur激活细胞内信号传递途径PI3-K/Akt有关。 |
| 关 键 词： | 心肌细胞 姜黄素 缺氧/复氧 凋亡 PI3-K/Akt |
Anti-apoptotic effects and mechanisms of curcumin on cardiomyocytes after hypoxia/reoxygenation injury |
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| Guo Bingyan,Han Rui,Shi Yinghui,Han Derong,Li Yongjun.Anti-apoptotic effects and mechanisms of curcumin on cardiomyocytes after hypoxia/reoxygenation injury[J].Modern Journal of Integrated Chinese Traditional and Western Medicine,2011,20(1):20-23. | |
| Authors: | Guo Bingyan Han Rui Shi Yinghui Han Derong Li Yongjun |
| Institution: | Guo Bingyan1,3,Han Rui2,Shi Yinghui3,Han Derong3,Li Yongjun1(1.The Second Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei,China,2.The First Hospital of Hebei Medical University,Shijiazhuang 050031,3.Hospital of Gucheng County,Gucheng 253800,China) |
| Abstract: | Objective It is to investigate the anti-apoptostic effect of curcumin(Cur) in cardiomyocytes after Hypoxia/Reoxygenation(H/R) injury and to explore its related mechanism.Methods Cardiomyocytes isolated from neonatal rats were cultured on plastic plates and were randomly divided into Control,H/R,H/R+Cur and H/R+Cur+LY294002 groups.Hypoxia/Reoxygenation injury was established by 4 hours of hyopxia and subsquently 1 hour of reoxygenation.The cell viability was measured with MTT.The apoptosis rate was determine... |
| Keywords: | PI3-K/Akt |
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