氯化镉对小鼠S180肉瘤的抑制作用及其对免疫功能的影响

目的：探讨氯化镉对S180肉瘤生长的抑制作用，寻找其最佳抗肿瘤应用剂量。方法：应用0.25、1.00及4.00 mg·kg^-1的氯化镉处理S180肉瘤小鼠模型，检测瘤重确定氯化镉对S180肉瘤的抑瘤作用，采用胸腺指数、脾脏指数、碳粒廓清试验、淋巴细胞转化实验、半数溶血素实验检测小鼠免疫功能的变化。结果：0.25、1.00及4.00 mg·kg^-1氯化镉组瘤重低于对照组（P< 0.05或P< 0.01），其中以1.00 mg·kg^-1剂量组最明显(P< 0.01)。0.25、1.00及4.00 mg·kg^-1氯化镉组模型鼠的胸腺指数及4.00 mg·kg^-1氯化镉组模型鼠的脾脏指数均高于对照组，0.25和1.00 mg·kg^-1氯化镉组模型鼠碳粒廓清指数、吞噬指数及淋巴细胞增殖率高于对照组(P<0.05或P<0.01)；各氯化镉处理组与对照组比较，半数溶血素数值差异无显著性(P>0.05)。结论：氯化镉能够抑制S180肉瘤生长，同时可增加荷瘤鼠非特异性及细胞免疫功能，以1 mg·kg^-1剂量最为明显。

Tumor suppressing and immune regulation effects of cadmium chloride in S180 sarcoma mouse models

Objective To discuss the tumor suppressing effect of cadmium chloride on S180 sarcoma,and to explore its favoriable dosage.Methods The mouse models bearing S180 sarcoma were treated with cadmium chloride with dosages of 0.25,1.00 and 4.00 mg·kg-1,the anti-tumor effect was evaluated by calculating of tumor weight;spleen and thymus indexes,carbon phage assay,lymphocyte transformed assay and hematolysis assay were used to measure the effect of cadmium chloride on immune function.Results Cadmium chloride with varied dosage of 0.25,1.00 and 4.00 mg·kg-1 inhibited the growth of S180 sarcoma,the tumor weights in cadmium chloride groups were significantly lower than that in control(P< 0.05 or P< 0.01),especially in 1 mg·kg-1 group(P< 0.01).The thymus indexes in 0.25,1.00,and 4.00 mg·kg-1 cadmium chloride groups and spleen index in 4.00 mg·kg-1 cadmium chloride group were increased compared with control group;the carbon phage capabilities and lymphocyte proliferation rates were higher in 0.25 and 1.00 mg·kg-1 cadmium chloride groups than those in control group(P< 0.05 or P< 0.01),the hematolysis assay had no difference between cadmium chloride groups and control group.Conclusion Cadmium chloride can inhibit the growth of S180 sarcoma as well as inhance the immune fuction.The favoriable dosage was 1 mg·kg-1.