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| 自噬增强结肠癌CD133~+细胞对5-FU的化疗抵抗 | |
| 引用本文： | 司晓丽,刘伟,杨爱军,王晨昱,李敏. 自噬增强结肠癌CD133~+细胞对5-FU的化疗抵抗[J]. 基础医学与临床, 2011, 31(7) |
| 作者姓名： | 司晓丽 刘伟 杨爱军 王晨昱 李敏 |
| 作者单位： | 兰州大学基础医学院,病理学研究所,甘肃省新药临床前研究重点实验室,甘肃,兰州,730000 |
| 摘 要： | 目的对5-FU诱导结肠癌CD133+细胞发生自噬的过程进行观察,初步探讨自噬对化疗的影响。方法用MTT法检测细胞增殖抑制率及IC50;用免疫磁珠分选CD133+细胞;用透射电子显微镜(TEM)观察细胞的超微结构,用MDC染色,荧光显微镜观察自噬囊泡;联合应用细胞活性实验、集落形成实验研究自噬对化疗的影响。结果不同浓度5-FU处理后,人结肠癌SW480细胞活性明显受到抑制,其半数抑制浓度IC50为(1.09±0.18)μg/mL。CD133+胞质出现大量的自噬体及自噬囊泡;相比较单独给予5-FU,联合给予5-FU及3-MA,CD133+细胞活性由(81.8±4.6)%下降到(56.3±5.5)%(P<0.05),集落形成率由(81.1±3.4)%下降(64.4±4.8)%(P<0.05)。结论 5-FU可诱导结肠癌CD133+细胞发生自噬;自噬的抑制能够增强5-FU对结肠癌CD133+细胞的细胞毒效应。 |
| 关 键 词： | 自噬 5-FU 结肠癌 CD133 磁珠细胞分选技术 |
Autophagy enhances chemotheragy resistance of CD133~+ colon cancer stem cells to 5-FU |
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| SI Xiao-li,LIU Wei,YANG Ai-jun,WANG Chen-yu,LI Min. Autophagy enhances chemotheragy resistance of CD133~+ colon cancer stem cells to 5-FU[J]. Basic Medical Sciences and Clinics, 2011, 31(7) | |
| Authors: | SI Xiao-li LIU Wei YANG Ai-jun WANG Chen-yu LI Min |
| Affiliation: | SI Xiao-li,LIU Wei,YANG Ai-jun,WANG Chen-yu,LI Min(Institute of Pathology,College of Basic Medicine,Lanzhou University,Gansu Key Laboratory for Preclinical Study of New Drugs,Lanzhou 730000,China) |
| Abstract: | Objective To observe the autophagy of CD133+ CSCs induced by 5-FU,and explore the effect of autophagy on the chemotherapy.Methods The cell proliferative inhibition rates and IC50 of 5-FU on human colon cancer cell lines SW480 were measured by MTT assay.CD133+ CSCs were purified with MACS(magnetic activated cell-sorting system) CD133 cell isolation kit.Then the morphplpgical features of cells were observed using transmission election microscopy(TEM),and the autophagic vacuoles(AV) was observed using fluoresc... |
| Keywords: | autophagy 5-fluorouracil colon cancer CD133 Magnetic activated cell sorting |
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