| HPLC法测定石楠藤中熊果酸、齐墩果酸的含量 |
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| 引用本文： | 吴建伟,张瑾. HPLC法测定石楠藤中熊果酸、齐墩果酸的含量[J]. 海峡药学, 2013, 25(9): 71-73 |
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| 作者姓名： | 吴建伟 张瑾 |
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| 作者单位： | 吴建伟 (江苏无锡药品检验所,无锡,214021)； 张瑾 (江苏无锡药品检验所,无锡,214021)； |
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| 摘 要： | 目的建立石楠藤有效成分的含量测定方法，提高江苏省习用中药材石楠藤的质量标准。方法采用HPLC法，以甲醇旬．1mol·L-1乙酸铵（85：15）为流动相，在210nm处检测石楠藤中的熊果酸、齐墩果酸的含量。结果熊果酸在0．0506—1．0120mg·mL“范围内呈良好的线性关系（r=0．9994，n=5）；加样回收率为98．05％，RSD为1．8％。齐墩果酸在在0．0534—1．0680mg·mLll范围内呈良好的线性关系（r=0．9994，n=5）；加样回收率为98．68％，RSD为1．O％。结论本方法操作简单、精密度、重现性较好，可用于控制石楠藤的质量。
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| 关 键 词： | 石楠藤 高效液相色谱 熊果酸 齐墩果酸 |
Determination of the contents of ursolic acid and oleanolic acid in Photinia serrulata by HPLC |
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| WU Jian-wei,ZHANG Jin. Determination of the contents of ursolic acid and oleanolic acid in Photinia serrulata by HPLC[J]. Strait Pharmaceutical Journal, 2013, 25(9): 71-73 |
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| Authors: | WU Jian-wei ZHANG Jin |
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| Affiliation: | (Jiangsu Wuxi Drug verification, Wuxi 214021, China) |
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| Abstract: | OBJECTIVE To establish a method to determine effective components in Photinia serrulata, and to improve its Quality standard. METHODS Using HPLC with the gradient elution of Methyl alcohol-0, l mol L-1 ammonium acetate solution（85： 15） detected the content of ursolic acid and oleanolic acid at 210nm. RESULTS Ursolie acid showed a good linear range in 0. 0506 ~ 1. 0120mg . mL-1 （r =0. 9994,n =5） ,recovery was 98.05% ; RSD was 1.8% ; and oleanolie acid showed a good linear range in 0. 0534 1. 0680rag mL- 1 （ r = 0. 9994, n = 5 ）, recovery was 98.68% ;RSD was 1.0%. CONCLUSION The method is simple, accurate and repeatable for the quality control for Photinia serrulata. |
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| Keywords: | Photinla serrulata HPLC Ursollc acid Oleanolic acid |
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