润坦注射液对大鼠局灶性脑缺血再灌注后神经元凋亡的影响

目的研究润坦对局灶性脑缺血再灌注的神经保护效应.方法制备线栓法大脑中动脉闭塞再灌注模型,干预组每天给予润坦3 mg/kg,对照组给予等量生理盐水,每日对大鼠神经功能缺损进行评分,于再灌注12 h、24 h、48 h和5 d处死动物,切片作TUNEL原位凋亡检测.结果润坦可以显著改善术后第2 d大鼠的神经功能缺损(P＜0.05),降低致死率,减少再灌注24 h以后的细胞凋亡数目(P＜0.05).结论润坦可以抑制大鼠脑缺血再灌注后神经元凋亡,对急性期脑梗死可能有神经保护效应.

The effect of vinpocetine on apoptosis in rats followed focal cerebral ischemia and reperfusion

Objective To study the neuroprotection of vinpocetine followed cerebral ischemia and reperfusion.Methods Reversible middle cerebral artery occlusion of cerebral ischemia model was applied in SD rats. Vinpocetine was given by 3mg/kg/day i.p., and the controls were given normal saline with same volume.Neurological scoring was evaluated every day in both groups.The amimals were killed and apoptosis was detected by TUNEL apoptosis kit at 12 h,24 h,48 h and 5 days after reperfusion.Results Vinpocetine could decrease the neurological scoring at the second day after operation( P < 0.05 ) and decrease the mortality significantly ( P < 0.01 ).Vinpocetine could decrease the numbers of apoptosis in 24 hours after reperfusion ( P < 0.05 ).Conclusion Vinpocetine can decrease the neurological deficit and inhibit apoptosis in rats followed cerebral ischemia and reperfusion.Neuroprotection of vinpocetine can be used in the treatment of acute cerebral infarction.