运用流式细胞术分析孕妇外周血中胎儿有核红细胞的比例与孕周的关系

目的 :探讨母亲外周血中胎儿有核红细胞 (NRBCs)的比例与孕周的关系。方法 :分离纯化孕 11～ 34周、2 1～ 35岁的 6 1例孕妇外周血中的胎儿细胞 ,采用荧光激活细胞分离技术 (FACS)分选表达转铁蛋白受体的阳性细胞 (CD71+ 细胞 )。将孕妇组、未孕组及新生儿脐血组进行对照。结果 :孕 11～ 34周孕妇外周血中CD71+ 细胞的比例为 (0 .35± 0 .2 5 )× 10 -2 ,与未孕组及新生儿脐血组差异均有高度显著性 (P <0 .0 1)。CD71+ 细胞在外周血中的比例在孕 17、18周达峰值 ,分别为 (0 .6 4± 0 .2 5 )× 10 -2 和 (0 .5 4± 0 .35 )×10 -2 。结论 :早、中、晚期孕妇外周血中存在胎儿有核红细胞。利用母血循环中的胎儿细胞进行产前诊断的最佳时间应在妊娠 17～ 18周

Frequency of fetal nucleated red blood cells in maternal peripheral and relationship to gestational age using flow cytometry

Objective:To investigate frequency of fetal nucleated red blood cells (NRBCs) in maternal peripheral blood and the realtionship to gestational age. Methods:Using fluorescence activated cell sorting (FACS),CD71 + cells were isolated from peripheral blood of 61 women of 11～34 week's gestation at the age of 21～35.The blood from nonparous women and the cord blood were compared as controls.Results: Frequency of CD71 + cells in maternal peripheral blood during 11～34 week's gestation was (0.35±0.25)×10 -2 .There were singnificant difference among the gravida and nonparous and newborn cord blood.Frequency of CD71 + cells was the highest at 17,18 week's gestation,(0.64±0.25)×10 -2 and (0.54±0.35)×10 -2 respectively. Conclusions:Fetal NRBCs are presented in peripheral blood of pregnant women during the first and second and third trimester of pregnancy.The best optimum time of prenatal diagnosis using fetal cell in maternal blood is at the 17～18 week of gestation.