丁酸钠激活TRAIL通路诱导白血病细胞凋亡机制的研究

本研究旨在探讨组蛋白去乙酰化酶抑制剂（histone deacetylase inhibitor，HDACI）丁酸钠诱导白血病细胞凋亡的机制。应用流式细胞术检测白血病细胞NB4、U937和Jurkat的凋亡情况，并应用Western blot和RT-PCR方法分别检测TRAIL及其受体DR4／DR5细胞表面蛋白及mRNA的变化情况。结果表明：经HDACI处理后，白血病细胞TRAIL和DR5蛋白及mRNA表达均随时间延长而增加，而DR4没有变化。结论：丁酸钠诱导白血病细胞凋亡的机制是与上调TRAIL及DR5mRNA的转录及蛋白表达密切相关的，而DR4可能不参与该凋亡过程。

Mechanism of Leukemia Cell Apoptosis Induced by Sodium Butyrate activating TRAIL pathway

This study was aimed to investigate the mechanism of leukemia cell apoptosis induced by histone deacetylase inhibitor（HDACI）. Flow cytometry was used to detect the apoptosis of leukemia cell lines NB4, U937 and Jurkat, and the changes of mRNA and protein expressions of TRAIL, DR4 and DR5 were detected by Western blot and RT-PCR respectively. The results showed that both TRAIL and DR3 protein and mRNA expressions in NB4, U937 and Jurkat cells increased after treated with sodium butyrate（ SB ） and in time-dependent manner. However, DR4 mRNA in leukemia cells was not significantly changed after treated with SB. It is concluded that the apoptosis mechanism of leukemic cell lines NB4, U937 and Jurkat induced by SB is closely related to the protein and mRNA expressions up-regulating TRAIL and DR5, but the DR4 may not participate in the apoptosis induced by SB.