人类精液中一氧化氮和尿酸含量检测的关系

目的探讨人精液中一氧化氮(N0)与尿酸含量的关系,对精了质量的影响。方法参照WHO标准方法,进行精液常规分析,按精子密度、活动率不同分为(正常、<20、20~40、>40)4个组。采用镀铜镉还原荧光法检测NO代谢产物硝酸盐(NO3-)。采用尿酸酶一过氧化物酶偶联法检测精液尿酸含量。结果70例不育组精液中尿酸含量和NO含量为(236.4±47.8)μmol/L、(78.7±1.6)μmol/L与正常生育组(398.6±52.3)μmol/L、(41.84±1.6)μmol/L呈显著性差异(P<0.01)。将尿酸含量与NO含量进行相关性分析,两者呈显著性负相关(r=-0.96,P<0.05)。不育各精子密度和活动率组精液尿酸含量随精子密度及活动率增加而上升,N0含量随之下降(P<0.01),结论精液尿酸含量测定可作为评价精子受活性氧损害的重要指标,证明尿酸对活性氧尤其在医学领域极为重视的NO损害精子具有保护性作用。

Nitric oxide level and human sperm uric acid in activity

Objective:To study the relationship between the nitric oxide(N0),and the uric acid activety of human sperm.Methods:Sperm routine analysis was operated uric acid refer to WHO standard method.Eighty-nine people were devided into 4 groups according the density and activity of sperm.A coppered reduced cadmium fluometric assay was used to detect nitrate,a metablic product of nitric oxide in sperm.The content of uric acid in semen was measured by uricase-peroxidase method.Results:The content of NO had an significantly negative relationship with the density,the movable rate,and uric acid activity of sperms in 70 patients.(r=-0.96,P<0.05).Conclusion:The detection of the activity of sperm uric acid is an effective index to appraise the capability of sperms being fertilized.NO inhibits the activity of sperm uric acid,and affects the maturity and movable activity of sperm.