芹菜素对氧糖剥夺损伤小胶质细胞的抗炎作用

目的探讨芹菜素对氧糖剥夺(OGD)损伤后复氧复糖(OGD/R)小胶质细胞中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)产生的影响。方法将原代培养的小胶质细胞随机分为正常组、二甲基亚砜(DMSO)组及芹菜素(10、25、50μmol/L)组,其中DMSO组和芹菜素组小胶质细胞进行OGD8h,之后加入相应药物培养基,观察24h后,收集细胞上清,用双抗夹心酶联免疫吸附法检测上清中IL-1β、TNF-α含量。结果24h后DMSO组细胞上清中IL-1β、TNF-α含量增多(P0.01)。芹菜素组TNF-α、IL-1β分泌量明显低于DMSO组。芹菜素可以抑制OGD后小胶质细胞的炎症反应,且呈剂量依赖性。结论芹菜素可以通过减少OGD/R小胶质细胞中IL-1β、TNF-α的产生而发挥神经保护作用。

Anti-inflammatory Effect of Apigenin on Microglia after Oxygen Glucose Deprivation and Reperfusion

Objective To study the neuroprotective effect of apigenin on microglia which was exposed to oxygen glucose deprivation and reperfusion （OGD/R）, which was characterized by its influence on IL-1β and TNF-α expression. Methods Primary microglial cultures were prepared from newborn rat brain. The purity of isolated cells were identified by GSA-IB4. The cells were randomized into 5 groups：normal group, DMSO group and apigenin-treated groups （10, 25, 50 μmol/L）. The cells of DMSO group and apigenin-treated group were exposed to 8 h of OGD and 24 h of reperfusion in the presence or absence of apigenin at a range of concentrations. Culture supernatants were collected and IL-1β and TNF-α were detected by ELISA assay. Results The expression of IL-1β and TNF-α were significantly higher in DMSO group （P〈0.01）, however, apigenin concentration-dependently suppressed IL-1β and TNF-α production of microglial. Conclusion Apigenin may play a neuroprotective effect which was related with depression of IL-1β and TNF-α production in OGD/R microglia.