Protection of chickens against hepatitis-hydropericardium syndrome and Newcastle disease with a recombinant Newcastle disease virus vaccine expressing the fowl adenovirus serotype 4 fiber-2 protein |
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| Affiliation: | 1. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, People’s Republic of China;2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, People’s Republic of China;1. Department of Pathobiology and Veterinary Sciences, University of Connecticut, Storrs, CT 06269, USA;2. Drug Discovery and Structural Biology Group, Health Biotechnology Division, NIBGE, Faisalabad, Pakistan;3. Department of Zoology, Government College University, Faisalabad, Pakistan;4. Vaccine Development Group, Animal Sciences Division, NIAB, Faisalabad, Pakistan;5. Institute for Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan;1. Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100094, China;2. College of Animal Science and Technology, Shandong Agricultural University, Tai''an, Shandong, 271018, China;3. College of Agriculture, Ningxia University, Yinchuan, 750021, China |
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| Abstract: | Newcastle disease (ND) is one of the most important and devastating avian diseases with considerable threat to the global poultry industry. Hepatitis-hydropericardium syndrome (HHS), caused by virulent fowl adenovirus serotype 4 (FAdV-4), is another highly infectious disease in chickens with severe economic impact. The effective way to combat ND and HHS is by vaccinating the poultry. In the present study, a recombinant NDV LaSota vaccine strain expressing full length fiber-2 gene of FAdV-4 (rLaSota-fiber2) was generated using reverse genetics. The FAdV-4 fiber-2 protein was expressed as a soluble form rather than NDV membrane-anchored form. The rLaSota-fiber2 was genetically stable, and it showed growth patterns in embryonated eggs comparable to that of parental rLaSota virus. Since our unpublished data demonstrated that delivery of live rLaSota-fiber2 in drinking water or ocular delivery of the vaccine didn’t produce protection against hypervirulent FAdV-4 challenge, even though the vaccine provide full protection against NDV challenge, the efficacy of the rLaSota-fiber2 was evaluated by delivering the vaccine intramuscularly in this study. Single-dose intramuscular vaccination of 2-week-old SPF White Leghorn chicks with the live or inactivated rLaSota-fiber2 provided complete protection against virulent NDV challenge. However, single-dose intramuscular vaccination with the live rLaSota-fiber2 vaccine provided better protection against virulent FAdV-4 challenge and significantly reduced faecal viral shedding comparing to the inactivated vaccine. These results indicate that the NDV-vectored FAdV-4 vaccine is a promising bivalent vaccine candidate to control both HHS and ND. |
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| Keywords: | Newcastle disease Hepatitis-hydropericardium syndrome Recombinant NDV LaSota vaccine strain Fowl adenovirus serotype 4 Fiber-2 |
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