|
|||||
|
|
| 1318nm Nd:YAG激光对体外胰腺癌细胞增殖的影响 | |
| 引用本文： | 刘建刚,刘志超,李哲.1318nm Nd:YAG激光对体外胰腺癌细胞增殖的影响[J].社区医学杂志,2007,5(17):1-2. |
| 作者姓名： | 刘建刚 刘志超 李哲 |
| 作者单位： | 1. 济宁医学院外科总论教研室 2. 济宁医学院皮肤病与性病教研室,山东济宁,272013 3. 泰安市中心医院肛肠病医院,山东泰安,271000 |
| 摘 要： | 目的研究高剂量1318 nm Nd:YAG激光的生物学效应。方法用波长为1318nm的Nd:YAG激光连续照射体外培养的处于对数生长期的人胰腺癌PC-3细胞,分组分别照射0、10、15、20、25、35、50 s,照射后,继续培养24小时,噻唑蓝比色法(MTT)测定各组OD值,研究细胞增殖情况。结果在一定剂量范围内,随着激光照射剂量的提高,PC-3细胞的OD值逐渐降低(P<0.05)。结论1318 nm Nd:YAG激光,在高剂量范围内照射PC-3细胞,具有明显抑制其增殖的作用,且呈剂量依赖性,对于指导临床应用具有重要价值。 |
| 关 键 词： | Nd:YAG激光 胰腺肿瘤 肿瘤细胞 比色法 |
Effect of high intensity 1318 nm Nd:YAG Laser irradiation on human pancreatic carcinoma cells (PC -3 ) proliferation in Vitro |
|
| LIU Jiangang,LIU Zhichao,LI Zhe.Effect of high intensity 1318 nm Nd:YAG Laser irradiation on human pancreatic carcinoma cells (PC -3 ) proliferation in Vitro[J].journal of community medicine,2007,5(17):1-2. | |
| Authors: | LIU Jiangang LIU Zhichao LI Zhe |
| Abstract: | Objective To investigate the biological effect of high intensity 1318 nm Nd:YAG laser on human pancreatic carcinoma cells(PC-3).Methods Seven groups of PC-3 cells were cultured in 96-well plates for 12 h and then irradiated with 1318nm Nd:YAG continuous wave laser for different time intervals.Cells were irradiated once and cultured for a next 24 h.MTT was used to assess the cell proliferation.Results A significant stimulative effect of 1318 nm laser on PC-3 cell proliferation was found(P<0.05).Conclusion The PC-3 cell proliferation can be inhibated by high intensity 1318 nm Nd:YAG continuous wave laser,which suggests that this laser could be beneficial to patients with pancreatic carcinoma. |
| Keywords: | Nd:YAG laser Human Pancreatic Carcinoma Cells(PC-3) MTT |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |