Tiam1表达与胃癌侵袭转移关系的临床和实验研究

目的检测T淋巴瘤侵袭转移诱导因子1(Tiam1)在正常胃黏膜组织、胃癌组织以及胃癌细胞系中的表达,探讨其与胃癌侵袭转移的关系。方法(1)应用免疫组化SABC法检测60例胃癌及正常胃黏膜组织中Tiam1蛋白的表达并分析其与胃癌临床病理参数间的关系。(2)应用逆转录PCR和SABC免疫组化技术分别检测Tiam1mRNA与蛋白在胃癌MKN45细胞(MO)及其高(MH)、低(ML)侵袭转移亚株中的表达,分析其与胃癌细胞侵袭转移能力的关系。结果(1)Tiam1蛋白在正常胃黏膜组织中阴性表达显著低于癌组织(0vs78.3%),两者差异有统计学意义(P<0.01),且随胃癌组织分化程度的降低、浸润深度的增加、TNM分期的升高及伴有淋巴结转移,Tiam1蛋白染色阳性率逐渐升高,差异有统计学意义(P<0.05)。但Tiam1蛋白表达水平与胃癌患者的性别、年龄、部位及癌肿大小无关,差异无统计学意义(P>0.05)。(2)Tiam1mRNA和蛋白在胃癌MKN45细胞高侵袭转移亚株中的表达均较其在MKN45细胞及低侵袭转移亚株中的表达为强,差异有统计学意义(P<0.05)。结论Tiam1表达与胃癌侵袭转移能力呈正相关,且其有可能成为反映胃癌恶性生物学行为的一种新型标志物。

Relationship between expression of Tiam 1 and invasion and metastasis of gastric cancer

Purpose To investigate the expression of T lymphoma invasion and metastasis inducing factor 1 (Tiam1) in tissues of normal gastric mucosa and gastric cancer, also its expression in gastric cancer cell lines, and to analyze the relationship between its expression and invasion and metastasis of gastric cancer. Methods The expression of Tiam1 protein in 60 cases of gastric cancer and their corresponding normal gastric mucosa was detected using strept avidin-biotin complex (SABC) immunohistochemical method. Simultaneously its relationship with the clinicopathological parameters of gastric cancer was also analyzed. RT-PCR and SABC immunohistochemical technology were also applied to detect the expression of Tiam1 mRNA and protein in gastric cancer cell lines, MKN-45 (M_O), its sublines with higher invasion and metastasis potentials (M_H) or with lower invasion and metastasis potentials (M_L). In addition, its relationship with the invasion and metastasis potentials of gastric cancer cells was analyzed. Results There was negative staining of Tiam1 protein in normal gastric mucosa, while the positive rate of Tiam1 protein in gastric cancer was 78.3% (47/60) (P< 0.01). With differentiated degree of histologic type descending, depth of invasion increasing, stage of TNM upgrading and lymph node metastasis happening, the positive staining rate of Tiam1 protein in gastric cancer significantly ascended (P<0.05), but there was no obvious correlation between its expression and the sex, age, location or size of gastric cancer (P>0.05). The expression of Tiam1 mRNA and protein in M_H was much higher than that in M_O and M_L (P<0.05), but there was no obvious difference between M_O and M_L (P>0.05). Conclusions Positive correlation exists between the expression of Tiam1 and invasion and metastasis of gastric cancer, which suggests Tiam1 may be acted as a new marker indicating its malignant biological behavior.