血管性痴呆小鼠海马NMDA受体mRNA表达特征及喜得镇的影响

目的观察喜得镇对血管性痴呆(vascular dementia,VD)小鼠海马神经元N-甲基D-天门冬氨酸(N-methyl-D-aspartate,NMDA)受体原位杂交变化的影响,并探讨NMDA受体原位杂交变化特征及喜得镇对其变化的影响.方法双侧颈总动脉结扎,反复缺血-再灌注法制备模型,药物组用喜得镇溶液灌胃,跳台试验和水迷宫试验观察其行为学改变,采用原位杂交技术观察小鼠海马神经元NMDA受体的表达变化.结果药物组小鼠学习、记忆成绩优于模型组(P＜0.01),其海马N-甲基-D-天门冬氨酸受体表达也明显增高(P＜0.01).结论喜得镇能改善血管性痴呆小鼠模型的学习、记忆功能,这可能与N-甲基D-天门冬氨酸受体mRNA水平正常化有关.

The effect of hydergine on N-methyl-D-aspartate receptor in neuron of hippocampus in mice with vascular dementia in situ hybridization

Objective To observe the effect of hydergine on N-methyl-Daspartate receptor mRNA in neuron of hippocampus in mice with vascular dementia. Methods The mice were subjected for reperfusion repeatedly on bilateral common carotid arteries by knots to establish the VD models. The behavioral abnormalities were investigated by step-down test and water maze test. Hydergine was given to the mice in medication group and N-methyl-D-aspartate receptor mRNA in neuron of hippocampus were observed in situ hybridization technique. Results Hydergine improved the grades of learning and memory in VD mice. The changes of N-methyl- D-aspartate receptor mRNA in neuron of hippocampus were ameliorated by hydergine. Conclusions Hydergine can improve dysfunction of learning and memory in VD, which suggest that hydergine may restore expression of N-methyl-Daspartate receptor mRNA.