宫颈鳞癌组织中PINCH和血管内皮生长因子C蛋白表达的研究

目的 探讨宫颈鳞癌组织中PINCH蛋白和血管内皮生长因子C(VEGF-C)蛋白的表达意义.方法 应用免疫组化SP法检测58例宫颈鳞癌、30名正常宫颈上皮组织中PINCH蛋白和VEGF-C蛋白的表达,并分析PINCH蛋白和VEGF-C蛋白的表达与临床病理特征的关系.结果 58例宫颈鳞癌组织中PINCH和VEGF-C的表达阳性率[分别为(62.1％ (36/58)和67.2％ (39/58)]高于正常宫颈上皮(0),差异有统计学意义(x2值分别为31.512、12.534,P均＜0.001).PINCH蛋白表达与年龄、肿瘤大小、肿瘤组织分化程度无明显相关(P均＞0.05),与有无淋巴结转移及临床分期相关(x2值分别为9.090、8.263,P均＜0.01).VEGF-C的表达与患者年龄、肿瘤大小无相关性(P均＞0.05),与有无淋巴结转移、肿瘤组织分级和临床分期相关(x2值分别为10.775、13.496、5.001,P均＜0.05).PINCH蛋白及VECF-C蛋白表达有关联(C =0.341,P＜0.01).结论 PINCH和VEGF-C可能协同参与了宫颈鳞癌的发生和发展过程,并在宫颈癌的侵袭和转移机制中发挥着重要作用.

Study on the expression of PINCH and vascular endothelial growth factor C in cervical squamous cell carcinoma

Objective To study the expression of PINCH and vascular endothelial growth factor C (VEGF-C) in cervical squamous cell carcinoma.Methods We detected the expression of PINCH and VEGF-C by immunohistochemistry SP in 58 cervical squamous cell carcinoma cases and 30 normal cervical epithelial tissue and analyzed their relationship to the clinical pathological features.Results The expression of PINCH and VEGF-C in 58 cervical squamous cell carcinoma(62.1％,36/58 ;67.2％,39/58 ) were higher than that in normal cervical epithelial tissue(0,0/30).The difference was significant( x2 =31.512,12.534,P ＜ 0.001 ).The expression of PINCH protein was not significantly associated with the age,tumor size and tumor differentiation grade ( P ＞ 0.05 ),but was associated with the lymph node metastasis and clinical stage ( x2 =9.090,8.236,P ＜ 0.001 ).The expression of VEGF-C had no significant correlationship with the age and tumor size( P ＞ 0.05 ) but had a correlationship with the lymph node metastasis,tumor differentiation grade and clinical stage( x2 =10.775,13.496,5.001,P ＜ 0.05 ).The expression of VEGF-C was correlated with the expression of PINCH protein( C =0.341,P ＜ 0.01 ).Conclusion It is possible that VEGF-C and PINCH take part in the development and progress of cervical squamous cell carcinoma and play an important role in the invasion and metastasis mechanism altogether.