欧猥迭宫绦虫谷胱甘肽转移酶1基因序列与功能的生物信息学分析

目的:预测欧猥迭宫绦虫成虫谷胱甘肽转移酶1(Glutathione S-Transferase1,GST1)基因及其编码蛋白的结构和功能。方法:应用生物信息学方法对从欧猥迭宫绦虫成虫cDNA文库中所获基因的氨基酸组成、基本性质进行分析,同时构建其编码蛋白的系统进化树并对其抗原表位进行预测及定位;建立其编码蛋白的三级空间结构模型。结果:GST1基因编码221个氨基酸,理论分子量为25767.39Da,理论等电点为5.98,理论分子式为C1188H1786N304O330S5,具有2个完整的保守功能域;在细胞内可能发挥基因表达调控及信号转导功能,与猪带绦虫的同源一致性达53%。在进化的过程中与绦虫类亲缘较近,而与脊椎类亲缘较远。预测最有可能的3个潜在抗原表位分别位于35-46aa、87-94aa、215-221aa之间。结论:GST1基因编码的蛋白为胞质型蛋白,可能是理想的药物作用靶点及新型免疫诊断的分子靶标,为对进一步研究奠定理论基础。

Application of bioinformatic analysis in sequences and functions of glutathione S-transferase1 (GST1) gene of Spirometra erinaceieuropaei

Objective:To analyze the structural characteristics of glutathione S transferase 1 gene from adult Spirometra erinaceieuropaei,and to predict the function of its encoded protein by applying the bioinformatics.Methods:Nucleic acid sequences of GST1 gene was obtained from cDNA library of Spirometra erinaceieuropaei adult worm,bioinformatics approach were applied to analyze GST gene's composition,the basic physical and chemical properties,multi-sequence homological alignment,tertiary structure of GST protein was established on its homology modeling.Results:GST1 gene encoded 221 amino acids,the theoretical molecular mass is 25767.39 Da,the theoretical isoelectric was 5.98,the theoretical formula is C1188H1786N304O330S5.There were two complete conservative domains,maybe play a role in the regulation of gene expression and signal transduction within the cell.Consistency of the screened gene with that of Taenia solium GST was 53%.In the process of evolution,it was close relative to the tapeworms but distant relative to the vertebrate.The three most likely potential antigenic epitopes are 35-46aa,87-94aa,215-221aa,which located out side of GST spatial structure and were far away from each other.Conclusions:It suggests that glutathione S transferase 1 of Spirometra erinaceieuropaei is predicted to be a cytoplasmic protein and may be have good application prospect for new immunodiagnostic molecular target,which provides the theoretical basis for further research.