复方中药对慢性萎缩性胃炎细胞凋亡及bcl-2、bax表达的影响

【目的】观察胃萎Ⅰ号治疗慢性萎缩性胃炎的临床效果，并从细胞凋亡和相关基因的表达方面探讨其作用机理。【方法】120例患者均经胃镜与病理检查证实为慢性萎缩性胃炎，分成两组，分别采用胃萎Ⅰ号、三九胃泰冲剂治疗，原位末端标记法（Tunel）检测细胞凋亡，免疫组化检测bcl-2、bax的表达。【结果】治疗组（胃萎Ⅰ号）综合疗效优于对照组（三九胃泰）（P〈0．05）。治疗组细胞凋亡率和bax表达率分别从21．81％、41．18％升高至40．52％、61．76％；而bcl-2表达率从85．71％下降至22．86％（P〈0．05）。对照组上述指标无明显变化。【结论】胃萎Ⅰ号能有效治疗慢性萎缩性胃炎，其机理可能是通过增强bax表达而抑制bcl-2表达，诱导胃黏膜细胞凋亡。

Effects of compound of traditional Chinese medicine on apoptosis and expression of bcl - 2 and bax of chronic atrophic gastritis cells

Objective] To study the effect of Weiweiyihao Granule on treating chronic atrophic gastritis and the mechanism of apoptosis and its relative genes expression. Methods] A total of 120 cases of chronic atrophic gastritis were divided into 2 groups, Chinese herbal observation group （treatment group） and Sanjiuweitai Granule control group （control group） . The apoptosis and expression of bcl-2 and bax were detected by Ttmel and immunohistochemical method separately. Results] The compre- hensive efficiency rate of treatment group was higher than that of control group （ P 〈 0.05） . Apoptosis and bax expression rate were significantly increased from 21.81%, 41.18% to 40.52%, 61.76% , but bcl-2 expression rate was decreased from 85.71% to 22.86 % in treatment group （ P 〈 0.05） . Control group didn＇t display obvious effect on apoptosis and genes expression. Conclusion ] Weiweiyihao Granule is effective for chronic atrophic gastritis , induces significant gastric epithelial apoptosis , increases bax expression but suppress bcl- 2 expression.