| 先天性巨结肠RET基因转录水平的研究 |
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| 引用本文： | 詹江华 房志勤. 先天性巨结肠RET基因转录水平的研究[J]. 中华小儿外科杂志, 1999, 20(1): 5-6 |
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| 作者姓名： | 詹江华 房志勤 |
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| 作者单位： | 天津市儿童医院外科 |
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| 摘 要： | 目的 探讨ＲＥＴ基因的表达情况与先天性巨结肠发生的关系。方法 采用ＲＴ－ＰＣＲ技术对１２例先天性巨结肠的狭窄段，移行段及扩张段分别做ＲＥＴ基因的表达，并以Ｇ３ＰＤＨ作内参标，观察ＲＥＴ基因的表达情况。结果 发现ＲＥＴ基因的表达从扩张段→移行段→狭窄段是逐渐减低的。结论 提示ＲＥＴ基因与先天性巨结肠的发生有一定的关系。
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| 关 键 词： | 先天性 巨结肠症 基因表达 聚合酶链反应 |
Expression of RET Proto-Oncogene in Hirschsprung's Disease |
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| ZHAN Jianghua,FANG Zhiqin,GU Jiqing. Expression of RET Proto-Oncogene in Hirschsprung's Disease[J]. Chinese Journal of Pediatric Surgery, 1999, 20(1): 5-6 |
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| Authors: | ZHAN Jianghua FANG Zhiqin GU Jiqing |
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| Abstract: | Objective To investigate the relationship between the expression of RET proto oncogene and the pathogenesis of Hirschsprung's disease. Methods The expression of RET proto oncogene in the aganglionic, transitional and dilated segments of colon from 12 children with Hirschsprung's disease was studied by RT PCR method. G3PDH was adopted as control.Results The expression of RET proto oncogene from the dilated segment to the transitional segment and aganglionic segment decreased gradually.Conclusions Expression of RET proto oncogene plays an important role in the pathogenesis of Hirschsprung's disease. |
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| Keywords: | Hirschsprung's disease Gene expression Polymerase chain reaction |
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