| 益肾健脾止血法对斑马鱼脑出血防治作用研究 |
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| 引用本文： | 傅华,曹左媛,徐旻,沈伟,唐靖一,周忠焱,肖颖,甘欣锦.益肾健脾止血法对斑马鱼脑出血防治作用研究[J].山西中医,2019(2):55-58. |
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| 作者姓名： | 傅华 曹左媛 徐旻 沈伟 唐靖一 周忠焱 肖颖 甘欣锦 |
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| 作者单位： | 上海中医药大学附属龙华医院 |
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| 基金项目： | 重大疑难疾病中西医临床协作试点:儿童急性白血病(国中医药办医政发);国家自然科学基金青年科学基金项目(编号:81603549);中医药文化背景下的医养结合示范工程建设(编号:A1-U1720501030101);上海市中医药事业发展三年行动计划(编号:ZY-(2018-2020)-FWtX-8001) |
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| 摘 要： | 目的:探索益肾健脾止血法对阿托伐他汀诱导的斑马鱼脑出血的影响。方法:用阿托伐他诱导Fli1∶EgFP/gAtA1∶dsRed双转基因斑马鱼脑出血,建立脑出血模型,分别给予不同浓度的三子补血汤,在荧光显微镜下拍照观察,计算斑马鱼脑出血面积和出血率。结果:与模型组相比,三子补血汤250、125μg/m L浓度组脑出血发生率有差异(P﹤0.05);与模型组相比,三子补血汤250、125、62.5、31.25μg/mL浓度组脑出血面积有差异(P﹤0.05)。结论:益肾健脾止血法可降低斑马鱼脑出血发生率,改善斑马鱼脑出血面积。
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| 关 键 词： | 脑出血 斑马鱼 益肾健脾止血法 实验研究 |
Prevention and treatment effect of Yishen Jianpi Zhixue therapy on cerebral hemorrhage of zebrafish |
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| Institution: | (Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China) |
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| Abstract: | Objective:To investigate the effect of Yishen Jianpi Zhixue therapy on zebrafish's cerebral hemorrhage induced by atorvastatin.Methods:The cerebral hemorrhage of Fli1:EGFP/GATA1:dsRed doubletransgenic zebrafish was induced by atorvastatin.The models of cerebral hemorrhage were established and given the different concentrations of Sanzi Buxue decoction respectively.Cerebral hemorrhage area and hemorrhage rate of zebrafish were observed and calculated by photograph under fluorescence microscope.Results:Compared with model group,the cerebral hemorrhagerates in groups of 250 and 125μg/mL concentrations of Sanzi Buxue decoction were different(P<0.05).Compared with model group,the cerebral hemorrhage areas in groups of 250,125,62.5 and 31.25μg/mL concentrations of Sanzi Buxue decoction were different(P<0.05).Conclusion:Yishen Jianpi Zhixue therapy can reduce the incidence ofcerebral hemorrhage of zebrafish,and improve the cerebral hemorrhage area of zebrafish. |
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| Keywords: | cerebral hemorrhage zebrafish Yishen Jianpi Zhixue therapy experimental research |
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