5-氮-2′-脱氧胞苷诱导膀胱癌EJ细胞凋亡研究

[目的]探讨5-氮-2′-脱氧胞苷（5-Aza-CdR）诱导膀胱癌EJ细胞凋亡机制。[方法]以不同浓度的5-Aza-CdR作用于膀胱癌EJ细胞，以四甲基偶氮唑蓝（MTT）法检测细胞的增殖程度，原位凋亡细胞检测技术（TUNEL）和流式细胞术（FCM）检测5-Aza-CdR对膀胱癌EJ细胞凋亡和细胞周期的影响。[结果]EJ细胞加入不同浓度的5-Aza-CdR（0.625，1.25，2.50，5.00mg/ml），与对照组相比各组EJ细胞增长速度减慢。与对照组相比，不同浓度的各组5-Aza-CdR（0.625，1.25，2.50，5.00mg/ml）可以显著降低EJ细胞的增殖比和增高EJ细胞的凋亡指数（P〈0.05），且与5-Aza-CdR呈浓度依赖关系（P〈0.05）；不同浓度的5-Aza-CdR作用EJ细胞后G0/G1期细胞明显增加（P〈0.05）。[结论]5-氮-2′-脱氧胞苷诱导膀胱癌EJ细胞凋亡并使EJ细胞阻滞于G0/G1期，从而抑制膀胱癌EJ细胞的增殖。

Study on EJ Cells'' Apoptosis Induced by 5-Aza-2''-decxycytidine (5-Aza-CdR)

[Purpose] To explore the mechanism on 5-Aza-2'-decxycytidine(5-Aza-CdR)inducing apoptosis in bladder cancer EJ cell line.[Methods] Cultured EJ cells were treated with variable doses of 5-Aza-CdR.The proliferation of EJ cell was detected by MTT;cell apoptosis and cell cycle of EJ cells were examined with TdT-mediated dUTP nick-end labeling(TUNEL)and flow cytometry(FCM).[Results] EJ cells were treated with different doses of 5-Aza-CdR displayed a slowly growth rate in contrast to the control group.5-Aza-CdR at certain concentration(0.625,1.25,2.50,5.00mg/ml)obviously decreased the proliferation ratio and increased the apoptotic index in EJ cells compared with those in control group(P<0.05).The inhibition effect of 5-Aza-CdR was dose-dependant(P<0.05).EJ cells treated with different doses of 5-Aza-CdR resulted in the cell cycle arrested at G0/G1(P<0.05).[Conclusion] 5-Aza-CdR induces cell apoptosis in bladder cancer EJ cell line and arrest the cell cycle at G0/G1,consequently causes cells proliferation inhibifed.