|
|||||
|
|
| 多重PCR检测铜绿假单胞菌耐药基因 | |
| 引用本文： | 何露,曾凡胜,范珍明,郭劲霞,黄烈,杜兆丰,陆学东. 多重PCR检测铜绿假单胞菌耐药基因[J]. 实用预防医学, 2011, 18(1): 12-14 |
| 作者姓名： | 何露 曾凡胜 范珍明 郭劲霞 黄烈 杜兆丰 陆学东 |
| 作者单位： | 1. 益阳医学高等专科学校,湖南,益阳,413000 2. 深圳市第四人民医院 |
| 基金项目： | 湖南省教育厅科学研究立项并资助项目 |
| 摘 要： | 目的了解铜绿假单胞菌耐药现状。方法采用多重PCR方法检测铜绿假单胞菌中的常见耐药基因。结果 39株铜绿假单胞菌耐药基因TEM、AmpC、VIM阳性率分别为84.6%、76.9%、17.9%,OXA基因扩增阴性。铜绿假单胞菌对替卡西林、妥布霉素、环丙沙星、头孢他啶耐药率均大于28.2%（11/39）,耐药率最高为庆大霉素59.0%,对多粘菌素E最敏感92.3%,本组菌株中有25.6%（10/39）表现为多重耐药。结论研究表明,携带TEM、AmpC、VIM是导致本组铜绿假单胞菌耐药的重要机制,β-内酰胺类抗生素耐药性及多重耐药性均较严重。 |
| 关 键 词： | 铜绿假单胞菌 多重PCR 耐药基因 |
Analysis on Resistance Genes of Antibiotics from Pseudomonas aeruginosa by Multiplex PCR |
|
| HE Lu,ZENG Fan-sheng,FAN Zhen-ming,et al.. Analysis on Resistance Genes of Antibiotics from Pseudomonas aeruginosa by Multiplex PCR[J]. Practical Preventive Medicine, 2011, 18(1): 12-14 | |
| Authors: | HE Lu ZENG Fan-sheng FAN Zhen-ming et al. |
| Affiliation: | HE Lu,ZENG Fan-sheng,FAN Zhen-ming,et al.(Yiyang Medical College,Yiyang 413000,Hunan,China) |
| Abstract: | Objective To investigate the resistance situation of Pseudomonas aeruginosa in Shenzhen. Methods The common antibiotic resistance genes in Pseudomonas aeruginosa were detected by multiplex PCR and E-test strips. Results TEM was detected in 39 stains(84.6%).Among them,VIM was found in 17.9%(7 stains) and AmpC in 76.9%(30 stains).The susceptibility results of antibiotics showed that the resistant rates of ticarcillin,tobramycin,ciprofloxacin,and ceftazidime were all beyond 28%.The resistant rate to gentamicin... |
| Keywords: | Pseudomonas aeruginosa Multiplex PCR Resistance genes |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |