雄激素及其受体在大鼠多囊卵巢综合征发病机制中的相互作用

目的:探讨雄激素及其受体在多囊卵巢综合征发病机制中的相互作用。方法:通过建立来曲唑所致PCOS大鼠模型,其中实验组灌胃服用来曲唑1 mg.kg-1.d-1,溶于1%羧甲基纤维素中,实验对照组灌胃服用1%CMC,空白对照组不给以灌胃刺激,连续灌服21天。同时用放射免疫方法测定大鼠血清中雄激素水平,应用Western Blot方法观察AR在子宫内膜中的表达。结果:实验组血清T浓度显著高于实验对照组及空白对照组(P<0.05),实验组AR水平明显低于实验对照组及空白对照组(P<0.05)。结论:雄激素及其受体在子宫内膜中的表达及其相互作用与PCOS的发病密切相关。

Interaction of androgen and its receptor in the pathogenesis of polycystic ovary syndrome in rats

Objective:To explore the interaction of androgen and its receptor in the pathogenesis of polycystic ovary syndrome(PCOS) in rats. Methods:The rat models of PCOS were established by letrozole,the rats in experimental group were treated by pouring letrozole(1 mg·kg-1·d-1 dissolved in 1% carboxymethylcellulose) into stomach for 21 days,the rats in experimental control group were treated by pouring 1% carboxymethylcellulose into stomach for 21 days,the rats in blank control group did not received any treatment.Radioimmunoassay was used to detect the serum level of androgen,Western Blot was used to observe the expression of androgen receptor in endometrium of rats. Results:The serum level of androgen in experimental group was significantly higher than those in experimental control group and blank control group(P<0.05),the serum level of androgen receptor in experimental group was significantly lower than those in experimental control group and blank control group(P<0.05). Conclusion:The expressions of androgen and its receptor and their interaction are related to the pathogenesis of PCOS closely.