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Presynaptic localization of B-50 phosphoprotein: the (ACTH)-sensitive protein kinase substrate involved in rat brain polyphosphoinositide metabolism
Authors:W.H. Gispen   J.L.M. Leunissen   A.B. Oestreicher   A.J. Verkleij  H. Zwiers  
Affiliation:1. Division of Molecular Neurobiology, State University of Utrecht, Utrecht, The Netherlands;2. Institute of Molecular Biology, State University of Utrecht, Utrecht, The Netherlands;3. Rudolf Magnus Institute for Pharmacology, State University of Utrecht, Utrecht, The Netherlands;1. Jiangsu Key Laboratory for Functional Substance of Chinese Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Key Laboratory Cultivation Base for TCM Quality and Efficacy, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China;2. Shanghai Institute of Materia Medica, University of Chinese Academy of Sciences, Shanghai 201203, China;1. Renewable Energies and Environment Department, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran;2. Hydrogen and Fuel Cell Laboratory, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran;3. Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran;1. Department of Aeronautical Engineering, Sathyabama Institute of Science and Technology, Chennai City, Tamil Nadu, India;2. Department of construction technology and management, Wollega University, Ethiopia;3. Department of Pediatrics, College of Medicine, King Saud University, [Medical City], King Khalid University Hospital, PO Box 2925, Riyadh 11461, Saudi Arabia;4. Department of Chemical Engineering, Kongu Engineering College, Perundurai, Erode 638 060, Tamil Nadu, India;5. Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam;6. Institute of Research and Development, Duy Tan University, Da Nang 550000, Viet Nam;1. Department of Biomedical Sciences, University of Sassari, Sassari, Italy;2. Department of Surgical, Microsurgical and Medical Sciences, University of Sassari, Sassari, Italy;3. Neurology Unit, «A. Segni» Hospital – ASL n. 1, Sassari, Italy;1. Institute and Outpatient Clinic for Occupational, Social and Environmental Medicine, University Hospital Munich (LMU), Munich, Germany;2. Department of Sport Medicine Humboldt University and Charité University School of Medicine, Berlin, Germany;3. Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for Lung Research, Munich, Germany;4. Department of Neurology, University Hospital Munich (LMU), Munich, Germany
Abstract:This study describes the ultrastructural localization in rat hippocampal tissue in situ and in isolated synaptosomes of the brain-specific phosphoprotein B-50, using affinity purified anti-B-50 immunoglobulins (IgGs). Evidence is presented for the presynaptic localization of B-50 in rat brain. Given this specific localization a model is presented outlining the presumed function of the B-50 protein in the membrane and describing possible neuromodulation by adrenocorticotropin hormone (ACTH)-like peptides.
Keywords:presynaptic localization   B-50 phosphoprotein   adrenocorticotropin hormone (ACTH)   polyphosphoinositide metabolism
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