益母草碱对急性心肌梗死大鼠的影响

目的:观察益母草碱对急性心肌梗死造成的心肌缺血和心肌细胞损害的影响并探讨其作用机制。方法:雄性SD大鼠随机分为5组,分别为假手术组,模型组,益母草碱高、低剂量组(30,15 mg·kg-1·d-1)和卡托普利阳性药组(4 mg·kg-1·d-1),除假手术组外其余各组采用结扎冠状动脉左前降支的方法制备大鼠急性心肌梗死模型,所有动物冠脉结扎前1周按相应剂量ip药物,第8天造模,术后继续ip给药2 d后,处死动物,2,3,5-氯化三苯基四氮唑(TTC)染色法检测并计算心肌梗死面积,酶联免疫吸附测定(ELISA)试剂盒测定大鼠血清中乳酸脱氢酶(LDH),肌酸激酶(CK)的水平,超氧化歧化酶(SOD)活性和丙二醛(MDA)含量。结果:与假手术组比较,模型组大鼠心肌梗死面积明显增大,血清中LDH,CK,MDA含量水平明显升高,总超氧化物歧化酶(T-SOD)水平明显降低(P0.05,P0.01);与模型组比较,益母草碱能明显减小心梗大鼠左心室梗死面积,并能明显降低心梗大鼠血清中LDH,CK及MDA的水平,明显升高血清中T-SOD的活性(P0.05,P0.01)。结论:益母草碱对急性心肌缺血造成的心肌损伤有良好的预防性保护作用,其作用机制可能与提高心肌组织抗氧化能力有关。

Effect of Leonurine on Rats of Acute Myocardial Infarction

Objective: To study the effect of leonurine on myocardial ischemia and myocardial cell injury caused by acute myocardial infarction, and its functional mechanism. Method: The model of acute myocardial infarction in rats was established through ligation of the left anterior descending coronary artery. Male SD rats were randomly divided into five groups:sham-operated group, leonurine high-dose (30 mg · kg^-1 · d^-1) group, low-dose (15 mg · kg^-1 · d^-1) group, positive control group and model group. One week before the coronary artery ligation, all groups, except for the sham-operated group, were given the corresponding dose of drugs. The model was built on the eighth day, and postoperative intraperitoneal administration lasted for 2 days. The animals were sacrificed, and myocardial infarct size was measured by TTC staining. The content of lactate dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD) and malondialdehyde (MDA) in rat serum were determined with enzyme-linked immuno sorbent assay (ELISA) kit. Result: Compared with the sham-operated group, the model group showed significant increase in myocardial infarct size, and serum LDH, CK and MDA content, and significant decrease in total superoxide dismutase (T-SOD) (P<0.05, P<0.01); compared with the model group, the leonurine group showed significant decrease in myocardial infarct size, and serum LDH, CK and MDA content, and significant increase in total superoxide dismutase (T-SOD) (P<0.05, P<0.01). Conclusion: Leonurine has a good protective effect on myocardial injury caused by acute myocardial ischemia. Its mechanism may be related to the improvement of the antioxidant capacity of myocardial tissues.