姜黄素对肝癌细胞株SMMC7721凋亡的研究

[目的]探讨姜黄素对人肝癌SMMC7721细胞株的生长凋亡作用及机制。[方法]用MTT法检测姜黄素对肝癌细胞系SMMC7721增殖的影响;应用流式细胞术检测不同浓度姜黄素对SMMC7721细胞凋亡及线粒体膜电位的影响。[结果]姜黄素对SMMC7721细胞具有明显的增殖抑制作用,在6.25～50μmol/L范围内成剂量依赖性,与对照组相比差异具有显著性意义,P〈0.01。姜黄素6.25、12.5、25、50μmol/L诱导SMMC7721细胞24 h的凋亡率为6.3%、7.2%、37.3%、72.3%,线粒体膜电位下降为6.3%、13.8%、47.8%和87.9%。[结论]姜黄素可通过诱导细胞凋亡而有效抑制肝癌细胞SMMC7721增殖,并与线粒体膜电位降低相关。

Apoptosis of liver cancer SMMC7721 cell line induced by curcumin

[Objective] This study is to investigate the effect of curcumin on growth and apoptosis of human liver canner SMMC7721 cell line and its mechanism.[Methods] The effect of curcumin on proliferation of cell line SMMC7721 was examined through MTT assay.The effect of curcumin on the apoptosis and chondrisome membrane potential of SMMC7721 cells were tested by flow cytometry.[Results] Curcumin inhibited the proliferation of SMMC7721 cells in a time and dose dependent manner(P<0.01).Concentrations of 6.25 to 50 μmol/L of curcumin for 24 hours induced apoptosis rate of SMMC7721 cells were 6.3%,7.2%,37.3% and 72.3% respectively,and decreased chondrisome membrane potential at 6.3%,13.8%,47.8% and 87.9% respectively.[Conclusion] Curcumin may induce apoptosis of SMMC7721 cells by inhibiting proliferation of the cells,which is possible to be related to the decreased chondrisome membrane potential.