原人参三醇组皂苷和人参皂苷-Re对MPP+诱导的SH-SY5Y细胞损伤的保护作用 |
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引用本文: | 李东薇,窦德强.原人参三醇组皂苷和人参皂苷-Re对MPP+诱导的SH-SY5Y细胞损伤的保护作用[J].张家口医学院学报,2011(5):1-7. |
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作者姓名: | 李东薇 窦德强 |
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作者单位: | 辽宁中医药大学药学院,大连,116620,中国 |
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摘 要: | 目的:探讨原人参三醇组皂苷(protopanaxatriol group,PPT)和人参皂苷-Re对1-甲基-4-苯基-吡啶阳离子(1-methyl-4-phenylpyridiniumion,MPP+)诱导的人多巴胺能神经母细胞瘤系SH-SY5Y细胞损伤的保护作用。方法:用不同浓度的PPT和人参皂苷-Re预处理细胞,以及不同体积分数的PPT和人参皂苷-Re含药血清预处理细胞,采用噻唑蓝(methylthiazolyldiphenyl-tetrazolium bromide,MTT)法检测细胞活力,计算细胞存活率,比较各组间的差异。结果:5μmol·L^-1和10μmol·L^-1的PPT和人参皂苷-Re均可以显著增加细胞存活率(与模型组比较,P〈0.05),体积分数10%的PPT和人参皂苷-Re含药血清能显著增加细胞存活率(与模型组比较,P〈0.001)。结论:PPT和人参皂苷-Re对MPP+诱导的SH-SY5Y细胞损伤有显著的保护作用,具有潜在的抗帕金森病作用。
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关 键 词: | 原人参三醇组皂苷 人参皂苷-Re SH-SY5Y细胞 帕金森病 神经保护 |
Protective Effect of PPT and Ginsenoside-Re in MPP+-treated SH-SY5Y Cells |
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Authors: | LI Dong-wei DOU De-qiang |
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Institution: | ( College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, 116620, China) |
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Abstract: | Objective:To investigate the protective effect of PPT (protopanaxatriol group) and ginsenoside-Re on l-methyl-4-phenylpyridiniumion (MPP+) -induced damage in SH- SY5Y (human dopaminergic neuroblastoma cell line) cells. Metbods:SH-SY5Y cells were treated with different concentrations of PPT,ginsenoside-Re or MPP containing serum. MTT colorimetry was used to test the viability of the cells and the survival rates were calculated and analyzed. Results:Cells treated by either 5 μmol·L^-1, 10 μmol·L^-1 PPT or ginsenoside-Re showed significantly higher viability as compared to control group (P〈0.05). In addition, 10% PPT treated or ginsenoside-Re medicated serum treated groups showed similar protective effect.Conelusion.PPT and ginsenoside-Re have neuroprotective effects in a cell model of Parkinson's disease MPP~ -induced SH-SYSY cell death, suggesting that both PPT and ginsenoside-Re may have anti-PD effect. |
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Keywords: | protopanaxatriol group ginsenoside-Re SH-SY5Y cells Parkinson's disease(PD) neuroprotective effects |
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