瘤果黑种草子总黄酮抗炎及平喘作用

目的：观察瘤果黑种草子总黄酮（TFNG）的抗炎及平喘作用。方法：选择昆明种小鼠、SD大鼠及健康豚鼠为实验动物，随机分为生理盐水组、阳性药对照组、TFNG低、高剂量组（小鼠100，400 mg·kg^-1，大鼠71.5，286 mg·kg^-1）。采用二甲苯致小鼠耳 廓肿胀、大鼠棉球肉芽肿实验观察总黄酮抗炎作用；采用整体动物小鼠喷雾致喘实验、离体豚鼠肺支气管灌流实验及离体回肠实验考察总黄酮的平喘作用。结果：TFNG 100，400 mg·kg^-1可明显抑制小鼠二甲苯所致的耳廓肿胀度，TFNG 71.5，286 mg·kg^-1可抑制大鼠棉球肉芽肿（P<0.01）；TFNG 100，400 mg·kg^-1可明显延长小鼠哮喘反应的潜伏期（P<0.01）；TFNG 3，6，12 g·L^-1可明显增加豚鼠肺灌流量，降低豚鼠回肠平滑肌收缩张力（P<0.01）。结论：瘤果黑种草子总黄酮具有一定的抗炎及平喘作用。

Anti-inflammatory and Asthma-relieving Effects of Total Flavones from Nigella glandulifera

Objective: To investigate the anti-inflammatory and asthma-relieving effects of total flavones from Nigella glandulifera (TFNG). Method: KM mice, SD rats and New Zealand rabbits were selected as experiment animal, randomly devided into saline control group, positive control group, TFNG low dosage and high dosage groups (mouse 100, 400 mg·kg^-1,rat 71.5, 286 mg·kg^-1). The mice external ear swelling induced by dimethyl benzene and rats cotton ball granuloma experiments were employed to evaluate the anti-inflammatory effect;asthma experiment induced by spraying the whole mice, isolated lung perfusion and isolated ileum smooth muscle experiments in guinea pigs were employed to evaluate the asthma-relieving effect. Result: TFNG 100, 400, 71.5 mg·kg^-1 could significantly inhibit dimethyl benzene-induced mice ear swelling and TFNG 286 mg·kg^-1 could siginificatey inbibit cotton pellet granuloma in rats(P<0.01);the low and high dosage (100, 400 mg·kg^-1) TFNG could significantly prolong the mice asthmatic response latency (P<0.01);the low, middle and high dosage TFNG (3, 6, 12 g·L^-1) could increase the perfusion of the isolated guinea pig lung, reduce the smooth muscle contraction of isolated guinea pig ileum significantly (P<0.01). Conclusion: TFNG has some anti-inflammatory and asthma-relieving effects.