山银花提取物抗动脉粥样硬化成分研究

目的对山银花中所含的四类化学成分进行提取,通过细胞和动物实验找出抗动脉粥样硬化作用相对较强的化学成分。方法对不同提取物,采用油红O染色观察对荷脂细胞中脂滴形成的影响,用高效液相色谱法检测荷脂细胞内胆固醇的含量;对apoE敲除小鼠主动脉标本作形态学检查,观察用药后血管内膜及斑块的变化。结果山银花提取物中四类化学成分均能不同程度地降低荷脂细胞内胆固醇含量。结论山银花中黄酮类成分具有较强抗动脉粥样硬化作用。

Study on the Anti-atherosclerosis Effect of Lonicera Macranthoides Hand Mazz. Extract

OBJECTIVE To extract four ingredients for medicine in Lonicera macranthoides Hand Mazz. and find an ingredient that has strong anti-atherosclerosis effect by cell and animal experiments. METHODS Rathonum red stain and high performance liquid phase analytic process were used to assess and detect the level of cholesterol. The shapes of aorta in apoE-deficent mice were examined and the changes of intimal and plaque were observed. RESULTS All of the four ingredients in Lonicera macranthoides Hand Mazz. can reduce cholesterol of dutch fat cells. CONCLUSION Flavonoids in Lonicera macranthoides Hand Mazz. have strong anti-atherosclerosis effect.