隐球菌性脑炎患者颗粒酶B、颗粒溶素、穿孔素的表达及临床意义

目的研究颗粒酶B（grmazymeB，GrB）、颗粒溶素（granulysin，GNLY）及穿孔素（pefforin，又称pore-formingpro—tein，PFP）三种免疫效应分子在隐球菌性脑膜炎（cryptococcalmeningitis）外周血单个核细胞中的表达及其临床意义。方法首先用密度梯度离心法分离得到25例隐球菌性脑膜炎患者和30例健康人外周血单个核细胞（peripheral blood monotmclearcells，PBMC），其次用免疫印迹（western blot）的方法检测其中的GrB、GNLY、及PFP的蛋白含量，用实时荧光定量（RVQ）-PCR方法测定其中GrB、GNLY、及PFP的mRNA含量。并分析隐球菌性脑膜炎患者蛋白含量与一些免疫指标的相关性。结果与健康对照组比较，隐球菌性脑膜炎患者PBMC中GNLY、PFP蛋白含量和mRNA均明显降低，而GrB无明显变化。隐球菌性脑膜炎患者中GNLY蛋白表达水平与血清IgG、IFN-γ水平成正相关（r=0．477，P〈0．05；r=0．534，P〈0．05），与IL-10水平成负相关（r=-0．505，P〈0．05）。结论GNLY、PFP）可能参与隐球菌性脑膜炎的疾病进程，这为探讨隐球菌性脑膜炎的病情监控和有效治疗提供了新的线索。

Expression of granzyme B,granulysin and perforin decreased significantly in peripheral blood mononuclear cells(PBMC)from patients with Cryptococcal Meningitis

Objective To investigate expression of granzyme B(GrB),granulysin(GNLY) and perforin perforin(PFP) in PBMC from patients with cryptococcal meningitis and the clinical significances.Methods Peripheral blood mononuclear cells(PBMC)from 25 patients with cryptococcal meningitis and 30 healthy controls were collected and the proteins of GrB,GNLY and PFP were assayed by western blot.Total RNA was extracted from these samples.Real time Fluorescence Quantitative Polymerase Chain Reaction(FQ-PCR) was performed to as...