大气颗粒有机提取物的染色体损伤作用研究

目的：探讨大颗粒有机提取物的染色体损伤作用，方法：使用高压液相色谱法对太原市高污染区太钢地区及低污染区阳曲地区大气总悬浮颗粒物有机提取物（EOM）中的部分多环芳烃（PAHs)进行成分分析，采用传统微核实验方法检测有机提取物所致小鼠的染色体损伤作用。结果：太原市太钢地区大气颗粒有机提取物中的多环芳烃含量明显高于阳曲地区，且BaP的含量在太钢地区达到0．819μg/m^3，超过阳曲地区10余倍，两地区EOM均可诱导小鼠微核率的升高，且存在剂量－反应关系，太钢组样品诱发小鼠微的作用明显高于阳曲组（P＜0．01），结论：大气颗粒有机提取物可致小鼠染色体损伤，其损伤程度可能与EOM中所含的PAHs种类和含量有关。

Study on Chromosomal Damage Induced by Organic Extracts of Air Particulates

ve To explore the chromosomal damage induced by organic extracts of air particulates. Methods Some components of polycyclic aromatic hydrocarbons (PAHs) in air particulates collected from highly air-polluted area, Taigang district, and lightly air-polluted area, Yangqu district, in Taiyuan, were analyzed by high pressure liquid chromatography. The chromosomal damage induced by organic extracts of air particulates was detected by traditional micronucleus test in mice. Results The contents of PAHs in organic extracts of air particulates in Taigang area were significantly higher than those in Yangqu area. The contents of benzo (a) pyrene [B(a)p] in air of Taigang area reached 0.819?g/m3, which was more than 10 time as high as that of Yangqu area. The organic extracts of air particulates in two areas both induced the increase of the frequencies of micronucleus of mice, and the dose-response relationships were observed. The mutagenecities of organic extracts of air particulates in Taigang area were significantly higher compared with those of Yangqu area (P<0.01) . Conclusion The organic extracts of air particulates could cause the chromosomal damages of mice, the degree of which was associated with the kinds and contents of PAHs in the organic extracts.