A simple method for ranking the affinities of monoclonal antibodies |
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| Authors: | V Van Heyningen D J Brock S Van Heyningen |
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| Affiliation: | 1. MRC Clinical and Population Cytogenetics Unit;2. University Department of Human Genetics, Western General Hospital, Edinburgh EH42XU;3. Department of Biochemistry, University of Edinburgh, George Square, Edinburgh EH8 9XD, u.K. |
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| Abstract: | ![]()
Measurement of the binding of constant trace amounts of labelled antigen by increasing dilutions of culture supernatant allows the ranking of monoclonal antibodies in the order of their affinity for the antigen. The theoretical basis for this method is discussed and it is illustrated with data from a set of anti-alphafoetoprotein monoclonal antibodies. Hybridomas secreting antibodies of desired affinity for immunoassay, histochemistry or antigen purification can thus be selected at an early stage after fusion. |
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| Keywords: | antibody affinity monoclonal antibody hybridoma alphafoetoprotein |
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