0.5 MPa高压氧暴露对培养的神经元中谷氨酸脱羧酶含量的影响

目的研究高压氧（HBO）暴露对体外培养的神经元中谷氨酸脱羧酶（GAD）的影响，为从细胞水平阐明急性氧中毒的发生机理提供依据。方法原代培养大鼠脑海马区的神经元，在不同体积的培养液中，经0．5MPa HBO暴露不同的时程，用免疫荧光法检测神经元内GAD含量的变化。结果0．5MPa HBO暴露可使神经元内GAD含量显著降低；并且减少培养液体积，同时缩短暴露时程，与正常培养液体积时长时程暴露可以达到相同效果。结论HBO暴露导致神经元内GAD减少，可能是急性氧中毒发生的重要原因之一；用体外实验方法研究氧中毒是完全可行的。

Effect of 0.5 MPa hyperbaric oxygen exposure on GAD content in cultured neurons

Objective To investigate the effect of hyperbaric oxygen (HBO) exposure on the content of glutamic acid decarboxylase(GAD) in cultured neurons,and to offer some scientific evidences at cell level to elucidate the mechanism of acute oxygen toxicity.Methods Primary cultured rat hippocampus neurons in different volume of medium were exposed to 0.5 MPa HBO for certain periods,and GAD content were detected by immunofluorescence staining.Results After exposed to 0.5 MPa HBO for a certain period,the GAD content in neuron body decreased significantly.When medium volume was decreased,the GAD content also decreased if the exposure period was shortened simultaneously.Conclusions HBO exposure could decrease GAD content in neurons,which may be an important factor correlative with the onset of acute oxygen toxicity.Our investigation also showed that it is completely feasible to investigate acute oxygen toxicity using in vitro methods.