帕瑞昔布对人胰腺癌细胞株BxPC-3、AsPC-1增殖凋亡的影响及其机制

目的:研究帕瑞昔布(parecoxib,PCB)对人胰腺癌细胞株BxPC-3和AsPC-1的增殖、凋亡及其可能的分子机制.方法:BxPC-3、AsPC-1细胞用不同浓度PCB的培养液孵育后,利用MTT法测定细胞活性,计算IC50值,TUNEL法检测处理后细胞的凋亡情况,RT-PCR验证相关蛋白的变化表达.结果:PCB对两种细胞生长呈时间和计量依赖性抑制;PCB处理后BxPC-3、AsPC-1两种细胞IC50值为:400.98μmol/L±10.78μmol/L、256.3μmol/L±2.98μmol/L;TUNEL法检测证明凋亡率增加;RT-PCR显示COX-2表达明显降低.结论:PCB可以抑制胰腺癌细胞增殖,并诱导其凋亡生长,其可能机制是通过抑制COX-2表达来实现的.

Parecoxib inhibits cell proliferation and induces apoptosis in human pancreatic cancer cell lines BxPC-3 and AsPC-1

AIM: To investigate the effect of parecoxib on cell proliferation and apoptosis in human pancreatic cancer cell lines BxPC-3 and AsPC-1 and to explore possible mechanisms involved. METHODS: After BxPC-3 and AsPC-1 cells were incubated with different concentrations of parecoxib, cell viability was measured by MTT assay to calculate the half maximal inhibitory concentration (IC50); cell apoptosis was evaluated by TUNEL assay; and the expression of COX-2 was detected RT-PCR. RESULTS: Cell viability was apparently inhibited by parecoxib in both cell types, and the inhibitory effect was timeand dose-dependent. The IC50 values in the two cell lines were 400.98 μmol/L ± 10.78 μmol/L and 256.3 μmol/L ± 2.98 μmol/L, respectively. Treatment with parecoxib increased apoptosis rate and down-regulated COX-2 expression in both cell lines. CONCLUSION: Parecoxib potently inhibits cell proliferation and induces apoptosis in human pancreatic cancer cell lines BxPC-3 and AsPC-1 possibly by suppressing the expression of COX-2.