^99mTc—HL9l肿瘤乏氧显像的动物实验

目的　用99mTc -HL91乏氧显像剂对肺腺癌小鼠模型进行体内分布和显像实验 ,以探索其对肿瘤显像的适用性 .方法　99mTc -HL91一步法标记 ,放化纯 >95 % .对 15只肺腺癌动物模型腹腔注射99mTc -HL9118 5MBq ,于 1～ 2 4小时处死取血液、肿瘤、脑、肺、心、肝、脾、肾、骨等标本称重 ,测定放射性计数 ,计算ID % /g及肿瘤组织与非肿瘤组织放射性比值 (T/NT) .同时在腹腔注射99mTc-HL91后于不同时间对模型进行静态显像 .结果　肿瘤组织对99mTc-HL91有较早较高的摄取和滞留 ,T/NT比值随时间延长而有增高趋势 .显像示肿瘤组织有较好的放射性浓聚 ,图像清晰 ,对比度良好 .结论　99mTc-HL91标记简单 ,标记率高 ,使用方便 ,在肿瘤组织中有较好的摄取与滞留 ,是一种良好的肿瘤乏氧显像剂 ,但需要进一步研究其机理和改进方法 ,提高T/NT比值 ,以开展临床试验

An Animal Experiment of Tumor Imaging with~(99m)Tc-HL91

Objective To elucidate the applicability 99m Tc-HL91,a hypoxic tracer to the imaging of hypoxia in tumors by animal experiment.Methods HL91 was labeled with 99m Tc by one step,and its chemical purity was >95%.15 mice with lung adenocarcinoma were used. 99m Tc-HL91(18 5MBq per mouse) was injected i p and animals were killed from 1h to 24h after injection.Blood,tumor,muscle,brain,heart,liver,spleen,kindey and bone samples were weighted and counted.The ID%/g and the ratio of tumor to nontumor (T/NT) were used to express the isotope retention.Besides,another mouse was used to take stationary imaging from 1h to 24h after intraperitonal injection with 99m Tc-HL91.Results The uptake and retention of HL91 in tumor was earlier and higher.The ratio of T/NT continued to increase until 24h.The tumor imaging was good and clear.Conclusions 99m Tc-HL91 is a convenient imaging tracer.Its labeling method is easy and labeling ratio is high.The uptake and retention of HL91 in tumor are higher.It is a good tracer in tumor hypoxia imaging.