下咽癌中基质金属蛋白酶-2的表达及其临床意义

目的 检测基质金属蛋白酶-2(MMP-2)在下咽癌及癌旁正常粘膜组织中的表达，探讨MMP-2与下咽癌组织生物学行为、转移及预后之间的关系。方法 运用免疫组化二步法检测32例下咽癌组织及癌旁组织中MMP-2抗体的表达，按不同的标准分组后进行统计学处理。结果 32例下咽癌组织中MMP-2蛋白表达阳性率为53．1％；癌旁正常粘膜组织与肿瘤组织中的阳性表达率比较、发生转移组与未发生转移组肿瘤组织中的阳性表达率比较均有显著性差异；染色阳性组3年生存率明显低于阴性组。结论MMP-2与下咽癌的转移有关，并且可能影响肿瘤患者的预后。

Expression of matrix metalloproteinase-2 in hypopharyngeal carcinoma and its clinical significance]

OBJECTIVE: To study the relationship between matrix metalloproteinase-2(MMP-2) and the biological behavior, metastasis and prognosis of hypopharyngeal carcinoma. METHODS: Two-step immunohistochemical method was used to examine the expression of MMP-2 mAb in 32 specimens of hypopharyngeal carcinoma tissues and adjacent normal mucosa. Statistical comparison was performed after grouping. RESULTS: It was found that the positivity rate for MMP-2 in the hypopharyngeal carcinoma tissues was 53.1%(17/32). Significant differences were noted when the expression levels of the mAb were compared between the normal mucosa adjacent to the carcinomas and the carcinomatous tissue, and between non-metastatic carcinomas and those with cervical lymph node metastasis. Patients with a positive result had obviously lower 3-year survival rate than those with negative results. CONCLUSION: MMP-2 may play a role in the lymph node metastasis of hypopharyngeal carcinoma, and is likely to be indicative of the patients' prognosis.