| 环孢菌素A两种不同产生菌原生质体的制备和再生 |
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| 引用本文： | 林永勉,郑孝贤,郑卫,程元荣. 环孢菌素A两种不同产生菌原生质体的制备和再生[J]. 中国抗生素杂志, 2007, 32(6): 337-340 |
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| 作者姓名： | 林永勉 郑孝贤 郑卫 程元荣 |
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| 作者单位： | 福建省微生物研究所,福州,350007 |
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| 摘 要： | 以环孢菌素A两种不同产生菌——雪白白僵菌和茄病镰刀菌为出发菌株，开展了原生质体制备和再生条件的研究，并分别实现了两个不同种的菌株原生质体再生。
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| 关 键 词： | 环孢菌素A 雪白白僵菌 茄病镰刀菌 原生质体 |
| 文章编号： | 1001-8689（2007）06-0337-04 |
| 修稿时间： | 2006-11-10 |
Formation and regeneration of protoplasts from two cyclosporin A producing fungus species |
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| Lin Yong-mian,Zheng Xiao-xian,Zheng Wei,Cheng Yuan-rong. Formation and regeneration of protoplasts from two cyclosporin A producing fungus species[J]. Chinese Journal of Antibiotics, 2007, 32(6): 337-340 |
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| Authors: | Lin Yong-mian Zheng Xiao-xian Zheng Wei Cheng Yuan-rong |
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| Affiliation: | Fujian Institute of Microbiology, Fuzhou 350007 |
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| Abstract: | Protocols for formation and regeneration protoplasts for two different fungus species, Beauveria nivea and Fusariurn solani, were described. High yield of protoplasts was obtained by using a combined enzyme system containing lywallzyme and snailase. The effects of factors such as osmotic stabilizers, cultiva- tion time, temperature, pH, incubation time on the formation and regeneration were studied. |
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| Keywords: | Cyclosporin A Beauveria nivea Fusarium solani Protoplast |
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