野生鲫鱼抗氧化水平作为水环境污染生物标志的研究

[目的]探讨用野生鲫鱼抗氧化水平作为生物标志来反映钱塘江水系有机物污染状况的可能性。[方法]以钱塘江水系中的闻堰、兰江将军岩和衢江樟潭为调查点,另选淳安街口作为本调查的对照点;对各个采样点表层水的化学需氧量(COD)、五日生化需氧量(BOD5)和苯系物的含量及野生鲫鱼肝超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量进行测定。[结果]樟潭(COD为87.8mg/L,BOD5为2.12mg/L)和闻堰(COD为87.8mg/L,BOD5为2.48mg/L)两调查点的COD和BOD5明显高于对照点(COD<10.0mg/L,BOD5为0.40mg/L),而将军岩点与对照点相比没有明显差异;苯系物均未超过GB3838—2002标准限值,但樟潭点苯、甲苯、氯苯、苯酚、硝基苯胺检测值明显高于对照点,其他指标浓度较低且4个点差别也不大。钱塘江各采样点野生鲫鱼肝重、鳃重、肝体比、鳃体比和GSH-Px活性[街口、将军岩、闻堰、樟潭分别为(0.13±0.07)、(0.73±0.25)、(2.47±1.09)、(1.81±0.80)U/mg蛋白]明显升高,或存在升高趋势;而SOD活性和MDA...

Study on Antioxidant Level in Wild Crucian as Biomarkers of Aquatic Environment Contamination

[Objective] To study aquatic contamination of organics in Qiantang River through the change of antioxidant level in wild crucian as biomarkers.[Methods] Chemical oxygen demand(COD),biochemical oxygen demand in 5 d(BOD5)and benzene series content in surface water,as well as superoxide dismutase(SOD)activity,glutathione peroxidase(GSH-Px)activity,and malondialdehyde(MDA)content in liver of wild crucian,all collected from Wenyan,Jiangjunyan and Zhangtan in Qiantang River valley were detected,and simutanously c...